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T. Sumioka, Y. Okada, P. S. Reinach, A. Inoue, M. Miyajima, S. Saika; Roles of Trpv1 Signal in Migration of Corneal Endothelium Epithelium. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6298.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the roles of TRPV1 receptor signaling in the migration of corneal endothelium and epithelium.
(1) Corneal epithelial wounds were made in TRPV1 -/- or +/+ mice (n=16) with 2mm trephine. The closure of the epithelial defect was determined by fluorescein staining after 6 to 42 h. (2) Corneal epithelial wounds were made in Wistar rat (n=96) with 2.5mm trephine. The eyes were immediately enucleated and incubated with TRPV1 receptor agonist (Capsaicin: 10 micro M) and/or antagonist (SB366791: 500 nM). The closure of the epithelial defect was determined by fluorescein staining after 6 to 36 h. (3) Japanese albino rabbits (n =36) were used. Blocks of central cornea (4 X 4 mm) were prepared. After partial scraping the endothelium to produce a defect, the blocks were organ-cultured for 24 hrs in Capsaicin: 10 micro M and/or SB366791: 500 nM.
(1) TRPV1-deficient suppressed the corneal epithelial wound closure. (2) Capsaicin stimulated the corneal epithelial and endothelial migration and SB366791 inhibit this migration-stimulating effect of capsaicin. (3) SB366791 also suppressed the corneal epithelial and endothelial migration.
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