April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Roles of Trpv1 Signal in Migration of Corneal Endothelium Epithelium
T. Sumioka; Y. Okada; P. S. Reinach; A. Inoue; M. Miyajima; S. Saika
Author Affiliations & Notes
  • T. Sumioka
    Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • Y. Okada
    Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • P. S. Reinach
    Biological Sciences, the State University of New York, College of Optometry, NY, New York
  • A. Inoue
    Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • M. Miyajima
    Laboratory Animal Center,
    Wakayama Medical University, Wakayama, Japan
  • S. Saika
    Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • Footnotes
    Commercial Relationships  T. Sumioka, None; Y. Okada, None; P.S. Reinach, None; A. Inoue, None; M. Miyajima, None; S. Saika, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 6298. doi:
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      T. Sumioka, Y. Okada, P. S. Reinach, A. Inoue, M. Miyajima, S. Saika; Roles of Trpv1 Signal in Migration of Corneal Endothelium Epithelium. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6298.

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Abstract

Purpose: : To investigate the roles of TRPV1 receptor signaling in the migration of corneal endothelium and epithelium.

Methods: : (1) Corneal epithelial wounds were made in TRPV1 -/- or +/+ mice (n=16) with 2mm trephine. The closure of the epithelial defect was determined by fluorescein staining after 6 to 42 h. (2) Corneal epithelial wounds were made in Wistar rat (n=96) with 2.5mm trephine. The eyes were immediately enucleated and incubated with TRPV1 receptor agonist (Capsaicin: 10 micro M) and/or antagonist (SB366791: 500 nM). The closure of the epithelial defect was determined by fluorescein staining after 6 to 36 h. (3) Japanese albino rabbits (n =36) were used. Blocks of central cornea (4 X 4 mm) were prepared. After partial scraping the endothelium to produce a defect, the blocks were organ-cultured for 24 hrs in Capsaicin: 10 micro M and/or SB366791: 500 nM.

Results: : (1) TRPV1-deficient suppressed the corneal epithelial wound closure. (2) Capsaicin stimulated the corneal epithelial and endothelial migration and SB366791 inhibit this migration-stimulating effect of capsaicin. (3) SB366791 also suppressed the corneal epithelial and endothelial migration.

Keywords: wound healing • cornea: epithelium • cornea: endothelium 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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