April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Evaluation of Corneal Healing, Limbal Progenitor Cells, and Vascularization in Wounded Pax 6+/+ and Pax 6+/- Mice
Author Affiliations & Notes
  • P. J. Accola
    Small Animal Medicine and Surgery,
    University of Georgia, College of Veterinary Medicine, Athens, Georgia
  • P. A. Moore
    Small Animal Medicine and Surgery,
    University of Georgia, College of Veterinary Medicine, Athens, Georgia
  • K. P. Carmichael
    Pathology,
    University of Georgia, College of Veterinary Medicine, Athens, Georgia
  • J. D. Lauderdale
    Cellular Biology, University of Georgia, Athens, Georgia
  • Footnotes
    Commercial Relationships  P.J. Accola, None; P.A. Moore, None; K.P. Carmichael, None; J.D. Lauderdale, None.
  • Footnotes
    Support  University of Georgia, College of Veterinary Medicine, Veterinary Ophthalmology Research Funds; Children's Glaucoma Foundation
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 6302. doi:https://doi.org/
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      P. J. Accola, P. A. Moore, K. P. Carmichael, J. D. Lauderdale; Evaluation of Corneal Healing, Limbal Progenitor Cells, and Vascularization in Wounded Pax 6+/+ and Pax 6+/- Mice. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6302. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Describe ocular pathology and compare corneal wound healing in Wild Type (WT) (Pax6+/+) and Small eye (Sey) (Pax 6+/-) mice.

Methods: : WT (n=84) and Sey (n=40) mice (age 2-3 months) were examined using biomicroscopy. Corneal wounding was performed by applying an n-heptanol saturated disk to OS for 60 seconds. Mice were evaluated on days 1, 2, 3 (if fluorescein positive on day 2), 4, 7, 14, and 28 post wounding. Each day, 3-5 mice were euthanized. Eyes were enucleated and placed in 4% paraformaldehyde. Immunohistochemistry (p63, sVEGFR-1) was performed. Days to negative fluorescein staining were compared using the Chi-Square test. The number of p63 staining basal cells was recorded and statistically evaluated by ANOVA. Staining for sVEGFR1 was recorded as positive or negative.

Results: : Anterior segments in all WT mice were normal. Pathology in Sey mice included corneal opacity (n=35), corneal vascularization (n=20), iris hypoplasia (n=36), and anterior cortical cataract (n=27). Application of n-heptanol resulted in removal of corneal epithelium in all mice. There was no significant difference in the amount of cornea wounded in WT and Sey mice (p>0.05). There was a statistically significant delay in corneal wound healing in Sey mice at days 2 and 3 when compared to WT mice (p<0.05). There was no significant difference in p63 staining (p>0.05). All mice exhibited comparable sVEGFR1 staining.

Conclusions: : The corneal healing delay in Sey mice does not appear to be due to a deficiency in p63 cellular expression. The comparable expression of sVEGFR1 suggests that it alone is likely not responsible for the corneal vascularization present in Sey mice.

Keywords: cornea: basic science • wound healing 
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