Influence of ErbB 2 Receptor on the Innervation of Corneal Stroma During a Wound Healing Process

Abstract

Purpose: : To characterize a neural-like phenotype of stromal cells following corneal injury and to stablish the role of ErbB 2 receptor during the reinnervation process of the cornea.

Methods: : PRK operated mice serve as model of corneal injury and wound healing process. Whole mounted corneas were incubated with antibodies against Neurofilament 200, GFAP, S100, NCAM, SMA and ErbB 2 receptor, in order to demonstrate the presence of these markers in the newly formed stromal tissue. Western Blot analysis were performed to evaluate the evolution of the expression of these markers.

Results: : After corneal injury, numerous cell processes expresses strongly the ErbB 2 receptor in the stroma. These cells accumulate preferently at the subepithelial layers. This profiles are closely related to axonal processes corresponding to regenerating axons and present a Schwann cell-like biochemical phenotype, as demonstrated by their immunoreactivity against GFAP and S100 protein.

Conclusions: : Axons could exert a potent effect over stromal cells and change temporarily their phenotype to improve reinnervation. This effect is mediated via ErbB 2 receptor activity.

Keywords: innervation: neural regulation • cornea: basic science • glia