Abstract
Purpose: :
To determine CD57+ expression on T cells stimulated with Dermatophagoides pteronyssinus (Der p) and to determine cell surface markers associated with central or peripheral memory on cells from conjunctivitis patients.
Methods: :
Mononuclear cells were obtained from peripheral blood to determine CD57, CD27, CD45RA, CD45RO, CCR7 and Ki-67 expression on cells before and after antigenic stimulation with Der p. Data were analyzed by flow cytometry. Mann-Whitney test was used to compare statistical differences. Significant correlation was performed with Pearson test. Significant differences were considered when p<0.05.
Results: :
We observed increased frequency of CD57 cells before (p=0.0001) and after (p=0.0003) Der p stimulation in conjunctivitis patients. CD45 isoforms index (CD45RA/CD45RO) was 3.3:1 before stimulation (p=0.02) and after stimulation was 3.1:1 (p=0.003). Positive correlations were observed when CD45RA was associated with CCR7 and CD27 (r=1; p<0.0001), negative correlations were observed when CD45RO was associated with CCR7 and CD27 (r=-1; p<0.0001).
Conclusions: :
Frequency of CD57 T cells increase after antigenic specific stimulation. We observed two cells subsets in conjunctivitis patients: one of them was CD27+CCR7+CD45RA+ (central memory), the other one was CD27-CCR7-CD45RO+ (peripheral memory). Functional studies are needed to elucidate the real role of these two subsets in patients with allergic conjunctivitis.Acknowledgments: CONACYT 71291
Keywords: cornea: basic science • inflammation • immunomodulation/immunoregulation