April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Exploration of a Suitable Induced Type-2 Diabetic Rodent Animal Model to Study Diabetic Ocular Complications
Author Affiliations & Notes
  • G. Reddy
    National Institute of Nutrition, Hyderabad, India
  • S. Palla
    National Institute of Nutrition, Hyderabad, India
  • S. Natarajan
    National Institute of Nutrition, Hyderabad, India
  • M. A. Patil
    National Institute of Nutrition, Hyderabad, India
  • P. Lopamudra
    National Institute of Nutrition, Hyderabad, India
  • N. V. Giridharan
    National Institute of Nutrition, Hyderabad, India
  • Footnotes
    Commercial Relationships  G. Reddy, None; S. Palla, None; S. Natarajan, None; M.A. Patil, None; P. Lopamudra, None; N.V. Giridharan, None.
  • Footnotes
    Support  Department of Science and Technology, Govt. of India
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 115. doi:
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      G. Reddy, S. Palla, S. Natarajan, M. A. Patil, P. Lopamudra, N. V. Giridharan; Exploration of a Suitable Induced Type-2 Diabetic Rodent Animal Model to Study Diabetic Ocular Complications. Invest. Ophthalmol. Vis. Sci. 2010;51(13):115.

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      © ARVO (1962-2015); The Authors (2016-present)

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Introduction: : Background: Prolonged exposure to chronic hyperglycemia during diabetes can lead to various complications including cataract and retinopathy. Type-2 diabetes (T2D) is the most common form of diabetes and it accounts for 90-95% of diabetic cases when compared to type-1 diabetes (T1D). Efforts have been on to understand the pathophysiology of diabetes-induced cataract and retinopathy in experimental animals. However, apart from genetic models, most of the studies are conducted on induced-T1D models. Considering the limitations of extrapolating T1D animal model data to T2D-induced diabetic complications, we have made an attempt to develop and evaluate a suitable animal model to study T2D-induced complications, mainly diabetic cataract and retinopathy.

Methods: : Evaluated neonatal-streptozotocin (nSTZ)-induced T2D in three different rat strains, WNIN, SD and WNIN-GR-Ob, by injecting STZ (45-90 mg/kg body weight) to two-day-old pups. Fasting blood glucose and insulin levels were monitored at regular intervals. Development of cataract and retinopathy was assessed by slit-lamp biomicroscope and indirect ophthalmoscope. Insulin resistance (IR) was assessed at regular intervals by performing oral glucose tolerance test (OGTT). After experimental period, the effect of IR and/ or hyperglycemia on biochemical changes in eye lens and retina were monitored.

Results: : Although, WNIN and SD rats showed IR and marginally increased levels of fasting glucose at the age of 3-4 months, they have neither developed frank hyperglycemia and nor cataract even after 8 months of STZ-injection. However, WNIN-GR-Ob rats developed hyperglycemia at the age of one month and showed a gradual increase in glucose levels up to three months and persisted till the end of the experimental period (five months). Interestingly, WNIN-GR-Ob rats have developed cataract by 3 months and showed signs of retinopathy by the age 5 months.

Conclusions: : Morphological, biochemical and molecular analysis supports that nSTZ- WNIN-GR-Ob rat could serve as model to study molecular basis of T2D-induced ocular complications and which may be useful to develop preventive/ therapeutic strategies.

Keywords: diabetic retinopathy • cataract • diabetes 

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