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S. S. VanderWyst, A. T. Read, D. R. Overby, W. D. Stamer; Patterned Expression of LM-511 and Integrin 6 in Human Schlemm’s Canal Endothelia. Invest. Ophthalmol. Vis. Sci. 2010;51(13):153. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Aqueous humor outflow resistance is generated near the juxtacanalicular connective tissue and the inner wall endothelium of Schlemm’s canal (SC). Due to a basal-to-apical directed pressure drop across these tissues, we hypothesize that SC cells must strongly adhere to their basement membrane through function-specific laminin heterotrimers and their cognate integrins. The purpose of this study was to characterize the laminin and laminin-specific integrins in Schlemm’s canal basement membrane.
Frozen sagittal sections through conventional outflow tissues from human donor eyes were probed with antibodies recognizing several monomers of laminin (laminin α5, β1, γ1, α3, β3, γ2) and laminin-specific integrin pairs (integrin α3, α6, β1, β4). En-face preparations of SC’s inner wall from perfusion-fixed eyes were immunostained for laminin and integrin co-localization. All tissue samples were examined with confocal microscopy and compared to controls. Mature SC cell monolayers were prepared for immunoprecipitation and Western blot to examine specific laminin heterotrimers.
Immunofluorescence, en-face imaging and Western blot show SC expression of LM-511 and integrin α6 in situ and in vitro. The patterned expression suggests that inner wall endothelia utilize integrin α6 to adhere to LM-511 in opposition to the force of basal-apical pressure gradient in vivo.
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