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M. N. Menke, M. Toeteberg-Harms, P. B. Knecht, V. Sturm, J. Funk; Reproducibility of Nerve Fiber Layer Thickness Measurements in Patients With Glaucoma and Healthy Controls Using Spectral OCT. Invest. Ophthalmol. Vis. Sci. 2010;51(13):228.
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© ARVO (1962-2015); The Authors (2016-present)
To assess the reproducibility of Spectral-Domain-OCT (SD-OCT) retinal nerve fiber layer thickness (RNFL) thickness measurements in healthy volunteers and patients with glaucoma and to compare these results to conventional Time-Domain-OCT (TD-OCT).
Fifty eyes of 25 healthy controls and 22 eyes of 11 patients with primary open angle glaucoma (POWG) were included. SD-OCT and TD-OCT circle scans were centered on the optic disc. In each eye OCT scans were performed 3 times by 2 independent observers. RNFL thickness was measured in 4 quadrants around the optic disc. In addition, the overall mean RNFL thickness was assessed. Intraclass correlation coefficients (ICC) and coefficients of variation (COV) were calculated. Inter-observer and inter-OCT reproducibility was visualized by using Bland-Altman analysis.
Intra-observer reproducibility for TD- OCT was good with an ICCmean RNFL thickness of 0.939 in controls and 0.98 in glaucomatous eyes. For SD-OCT, intra-observer reproducibility was higher with an ICC of 0.989 for controls and 0.997 for glaucomatous eyes.COVs for TD-OCT ranged from 2.9% to 7.7% in controls and from 6.0% to 13.3% in glaucoma patients. COVs for SD-OCT ranged from 0.3% to 1% in controls and from 0.9% to 2.3% in glaucomatous eyes.COVs were influenced by various factors. In the glaucoma group COVs were significantly higher (p<0.0001) compared to controls. COVs increased by a mean of 5.1% when TD-OCT was used instead of SD-OCT (p<0.0001).In controls, TD-OCT showed a significant tendency to measure higher RNFL values in the inferior quadrant compared to SD-OCT (mean difference + 4.7 µm, p<0.0001). In the glaucoma group, SD-OCT showed a significant tendency to measure higher RNFL values in mean RNFL thickness, and the superior (mean difference +8.2 µm, p<0.0001), temporal (mean difference +7 µm, p<0.0001), and inferior quadrant (mean difference +3.5 µm, p=0.017) compared to TD-OCT.
SD-OCT RNFL thickness measurements in healthy volunteers and glaucoma patients showed good intra- and inter-observer reproducibility. Especially in glaucomatous eyes, reproducibility was superior to TD-OCT.
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