April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Reproducibility of Nerve Fiber Layer Thickness Measurements in Patients With Glaucoma and Healthy Controls Using Spectral OCT
M. N. Menke; M. Toeteberg-Harms; P. B. Knecht; V. Sturm; J. Funk
Author Affiliations & Notes
  • M. N. Menke
    Department of Ophthalmology, University of Bern, Bern, Switzerland
    Department of Ophthalmology, University of Zurich, Zurich, Switzerland
  • M. Toeteberg-Harms
    Department of Ophthalmology, University of Zurich, Zurich, Switzerland
  • P. B. Knecht
    Department of Ophthalmology, University of Zurich, Zurich, Switzerland
    Department of Ophthalmology, Kantonsspital Luzern, Luzern, Switzerland
  • V. Sturm
    Department of Ophthalmology, University of Zurich, Zurich, Switzerland
  • J. Funk
    Department of Ophthalmology, University of Zurich, Zurich, Switzerland
  • Footnotes
    Commercial Relationships  M.N. Menke, None; M. Toeteberg-Harms, None; P.B. Knecht, None; V. Sturm, None; J. Funk, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 228. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Reproducibility of Nerve Fiber Layer Thickness Measurements in Patients With Glaucoma and Healthy Controls Using Spectral OCT
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      M. N. Menke, M. Toeteberg-Harms, P. B. Knecht, V. Sturm, J. Funk; Reproducibility of Nerve Fiber Layer Thickness Measurements in Patients With Glaucoma and Healthy Controls Using Spectral OCT. Invest. Ophthalmol. Vis. Sci. 2010;51(13):228.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : To assess the reproducibility of Spectral-Domain-OCT (SD-OCT) retinal nerve fiber layer thickness (RNFL) thickness measurements in healthy volunteers and patients with glaucoma and to compare these results to conventional Time-Domain-OCT (TD-OCT).

Methods: : Fifty eyes of 25 healthy controls and 22 eyes of 11 patients with primary open angle glaucoma (POWG) were included. SD-OCT and TD-OCT circle scans were centered on the optic disc. In each eye OCT scans were performed 3 times by 2 independent observers. RNFL thickness was measured in 4 quadrants around the optic disc. In addition, the overall mean RNFL thickness was assessed. Intraclass correlation coefficients (ICC) and coefficients of variation (COV) were calculated. Inter-observer and inter-OCT reproducibility was visualized by using Bland-Altman analysis.

Results: : Intra-observer reproducibility for TD- OCT was good with an ICCmean RNFL thickness of 0.939 in controls and 0.98 in glaucomatous eyes. For SD-OCT, intra-observer reproducibility was higher with an ICC of 0.989 for controls and 0.997 for glaucomatous eyes.COVs for TD-OCT ranged from 2.9% to 7.7% in controls and from 6.0% to 13.3% in glaucoma patients. COVs for SD-OCT ranged from 0.3% to 1% in controls and from 0.9% to 2.3% in glaucomatous eyes.COVs were influenced by various factors. In the glaucoma group COVs were significantly higher (p<0.0001) compared to controls. COVs increased by a mean of 5.1% when TD-OCT was used instead of SD-OCT (p<0.0001).In controls, TD-OCT showed a significant tendency to measure higher RNFL values in the inferior quadrant compared to SD-OCT (mean difference + 4.7 µm, p<0.0001). In the glaucoma group, SD-OCT showed a significant tendency to measure higher RNFL values in mean RNFL thickness, and the superior (mean difference +8.2 µm, p<0.0001), temporal (mean difference +7 µm, p<0.0001), and inferior quadrant (mean difference +3.5 µm, p=0.017) compared to TD-OCT.

Conclusions: : SD-OCT RNFL thickness measurements in healthy volunteers and glaucoma patients showed good intra- and inter-observer reproducibility. Especially in glaucomatous eyes, reproducibility was superior to TD-OCT.

Keywords: imaging/image analysis: clinical • clinical research methodology • ganglion cells 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept