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A. Shiraishi, T. Kobayashi, Y. Hayshi, Y. Ohashi; Murine Epidermal Cells Can be Transdifferentiated Into Corneal Epithelial-Like Cells in the Corneal Epithelial Environment. Invest. Ophthalmol. Vis. Sci. 2010;51(13):357.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the potential for murine epidermal cell transdifferentiation into corneal epithelial-like cells.
use epidermal cells (MKCs) were isolated from K12Cre/ZEG mice which expressed GFP (green fluorescent protein) induced by a cornea-specific keratin 12 gene. The MKCs were cultured in serum free medium (CnT-07). Equal numbers of MKCs and normal mouse corneal epithelial cells (MCEs) from wild type mice were mixed and seeded (1x104/cm2) onto plastic, an amniotic membrane (AM), or an epithelium-denuded WT mouse cornea. The cells were cultured in serum free medium (CnT-30) with 0.4 mM Ca or SHEM (5% FBS) for 4 days and examined for GFP expression by fluorescence microscopy.
GFP expression was detected in MKCs cultured on the epithelium-denuded mouse cornea with either CnT 30 or SHEM; however no GFP was detected in MKCs cultured on the plastic or AM. The GFP positive cells were predominantly detected in the corneal limbal region.
The present study demonstrates that MKCs have the potential to transdifferentiate into corneal epithelial-like cells. The results indicate that transdifferentiation may require the effect of coculture with corneal epithelial cells, as well as the corneal limbal environment.
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