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S. S. Chaurasia, A. K. Riau, T. T. Wong, L. Tong; Altered Corneal Wound Healing in Transglutaminase 2 (TG2-/-) Deficient Mice. Invest. Ophthalmol. Vis. Sci. 2010;51(13):360.
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Transglutaminase 2 (TG2) plays a multifunctional role during tissue repair to modulate cell-matrix interactions, cell adhesion, collagen synthesis, extracellular matrix restructuring and organization. We propose to evaluate the role of TG2 during corneal epithelial wound healing in TG2 deficient (TG2-/-) mice.
4-6 weeks old wild type (WT) and transglutaminase 2 (TG2-/-) deficient mice with C57BL/6 background were selected for this study. WT and TG2-/- animals were divided into five groups and sacrificed after days 1, 2, 3, 5, and 7 to determine the rate of corneal epithelial wound closure. Each group contained 6 animals and only one eye from each animal was used for experiments. Epithelial injury was achieved with the use of a #64 Beaver blade scraping the entire central cornea except for a 0.5 mm rim at the limbus. Presence of collagen I, TG2, laminin, fibronectin, and SPARC expression were evaluated by immunohistochemistry and western blot analysis. In situ zymography was performed to localize the gelatinase activity in cornea.
Rate of re-epithelialisation in the TG2-/- mice was significantly slower than WT. The expression of collagen, fibronectin, laminin, and SPARC were upregulated in concert with the initial stages of wound healing, but decreased after complete re-epithelialisation. In situ zymography showed increased gelatinolytic activity during the initial phase of wound healing.
TG2 appears to play a central role in the expression and activities of various key wound healing molecules involved in corneal epithelial injury.
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