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K. T. Vanichsarn, D. C. Tran, Z. Deng, H. P. Truong, M. Ficinski, C. Ju, M. Lii, H. Sun, L. Yu, E. K. Wong, Jr.; Examinations of Corneal Wound Healing Rates Using Optical Coherence Tomography Imaging. Invest. Ophthalmol. Vis. Sci. 2010;51(13):373.
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To observe the differences in corneal healing rates between eyes that have been treated to replicate symptoms of neurotrophic keratopathy and normal eyes via optical coherence tomography imaging. This can aide in the development of a wound healing model associated with neurotrophic keratopathy and keratoconjunctivitis sicca.
Using radiofrequency ablation, the trigeminal nerve of the Sprague-Dawley rats were denervated at the V1-V2 junction. The consequences of the procedure were confined to the left eye, preserving the right eye as the control. Within three to five days after surgery, femtosecond laser was used to lesion the corneal surface, 1.500mm in diameter and 0.300mm in depth. To examine corneal changes, optical coherence tomography (OCT) imaging was performed on both eyes of each rat on the third and fifth day after the lesion treatment. The location of the lesion was determined by looking at the three-dimensional composite of the OCT images for each eye. The slides that exemplified the lesion were analyzed for textural surface differences. The changes in corneal surface induced by the femtosecond lesion were measured in comparison to the surface of a normal cornea by ImageJ. The mean percent change of the lesion compared to the normal cornea over from the third to the fifth day was calculated to compare the rate of corneal healing.
Three to five days after trigeminal ablation, the control eye exhibits a significantly greater rate of healing than the treated eye (p=0.001827). After laser treatment, the corneal surface of the lesion of all the eyes showed an increase in surface area. Healing is implied by a decrease in the proportion of the corneal lesion surface to the control surface, from the third day compared to the fifth day. The proportion of the control eye decreased by 0.1022 (10.22%) while the proportion of the corneal surface are of the treated eye decreased by 0.0133 (1.33%).
These results maintain the concept of reduced corneal healing in neurotrophic keratopathy-treated rats. The implications of this project provide a precise imaging technique for the characterization of the corneal healing process to aid in the development of treatments for damaged neurotrophic keratopathic corneas.
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