April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Expression of Claudins in the Normal Adult Human Retinas
Author Affiliations & Notes
  • W. Xiao
    Sun Yat-sen University, Zhongshan Ophthalmic Center, Guangzhou, China
  • Y. Luo
    Sun Yat-sen University, Zhongshan Ophthalmic Center, Guangzhou, China
  • X. Chen
    Sun Yat-sen University, Zhongshan Ophthalmic Center, Guangzhou, China
  • J. Li
    Sun Yat-sen University, Zhongshan Ophthalmic Center, Guangzhou, China
  • X. Ding
    Sun Yat-sen University, Zhongshan Ophthalmic Center, Guangzhou, China
  • M. Zeng
    Sun Yat-sen University, Zhongshan Ophthalmic Center, Guangzhou, China
  • S. Tang
    Sun Yat-sen University, Zhongshan Ophthalmic Center, Guangzhou, China
  • Footnotes
    Commercial Relationships  W. Xiao, None; Y. Luo, None; X. Chen, None; J. Li, None; X. Ding, None; M. Zeng, None; S. Tang, None.
  • Footnotes
    Support  the National Natural Science Foundation of China(30872819)
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 40. doi:
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    • Get Citation

      W. Xiao, Y. Luo, X. Chen, J. Li, X. Ding, M. Zeng, S. Tang; Expression of Claudins in the Normal Adult Human Retinas. Invest. Ophthalmol. Vis. Sci. 2010;51(13):40.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Tight junctions (TJs) are important components of the blood-retinal barrier. However, the evidence for the presence of tight junction-associated proteins in human retina is lacking. To understand the contribution of each claudin subtype to TJ formation in the inner blood-retinal barrier of human eye, the expression and distribution of claudin subtypes in adult human retina were investigated in this study.

Methods: : Eye balls of healthy adult individuals were obtained from the Eye Bank of Zhongshan Ophthalmic Center. Whole retinas were dissected carefully. RT-PCR was used to assess mRNA expression of claudins in normal adult human retinas. Western blot analysis and immunofluorescence staining were performed to determine the protein expression and tissue distribution of selected claudins respectively.

Results: : RT-PCR showed that claudin-12 was the most abundant subtype, followed by claudin-1, -2, -3, -4, -5 and -22. Claudin-6 to -11, -14 to -19 and -23 were not detected at mRNA level. Expression of claudin-1, -2, -3, -4 and -5 were identified in the retinas by western blot. By immunofluorescence staining, claudin-1, -2, -3 and -5 were found to be localized on retinal microvessels.

Conclusions: : This study indicates that claudin-1, -2, -3, -4 and -5 are expressed on the retinal vessels in the normal adult human retinas. These claudins may be responsible for maintaining the normal function of inner blood-retinal barrier.

Keywords: retina • cell adhesions/cell junctions • vascular occlusion/vascular occlusive disease 
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