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K. M. Nassar, E. El Far, O. Tatar, J. Lueke, M. Lueke, A. Tura, S. Grisanti; Macrophage Activation Pattern in Human Choroidal Neovascular Membranes. Invest. Ophthalmol. Vis. Sci. 2010;51(13):401.
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In the present study we aim to characterize the pattern of macrophage activation in human choroidal neovascular membrane (CNV) following anti-VEGF and/or photodynamic therapy (PDT).
39 surgically excised CNV were stained using CD68, CCR7 and CD163 antibodies staining for pan macrophages, classically activated macrophage (M1 macrophages) and alternatively activated macrophage (M2 macrophages); respectively. The immunopositive cell density was calculated per mm2 in 400X images. M1/M2 ratio was calculated. Paired sample test and unpaired t test was used. The level of significance was assumed if p <0.05.
In hemorrhagic CNV a selective shift of the macrophage activation towards the alternatively activated macrophages was detected. Hemorrahgic CNV had a significantly higher M2 macrophage compared to both classic and occult CNV. PDT treated CNV showed an overall reduction of pan macrophages with selective reduction of M2 cells causing a shift towards classic macrophage activation pattern compared to controls. Adjuvant use of avastin caused a reduction of both M1 and M2 subtypes compared to controls and an increase pan macrophages compared to PDT monotherapy treatment.
Macrophages play major but conceivably also different roles in CNV development. The identification of different activation patterns may be important for distinct therapeutic strategies.
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