April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Dyalizable Leukocyte Extracts and TLR Agonist Effects on VEGF Secretion by Human Limbal Epithelial Cells
S. Groman-Lupa; L. Vizuet-Garcia; G. A. Luna-Baca; A. Robles-Contreras; E. Rivera-Mendez; J. Serafin; I. Estrada-Garcia; S. Estrada-Parra S; M. Perez-Tapia; M. C. Jimenez-Martinez
Author Affiliations & Notes
  • S. Groman-Lupa
    Department of Immunology/Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • L. Vizuet-Garcia
    Department of Immunology/Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • G. A. Luna-Baca
    Department of Immunology/Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • A. Robles-Contreras
    Department of Immunology/Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • E. Rivera-Mendez
    Department of Immunology, Laboratory of Molecular Immunology II, National School of Biological Sciences, Mexico City, Mexico
  • J. Serafin
    Department of Immunology, Laboratory of Molecular Immunology II, National School of Biological Sciences, Mexico City, Mexico
  • I. Estrada-Garcia
    Department of Immunology, Laboratory of Molecular Immunology II, National School of Biological Sciences, Mexico City, Mexico
  • S. Estrada-Parra S
    Department of Immunology, Laboratory of Molecular Immunology II, National School of Biological Sciences, Mexico City, Mexico
  • M. Perez-Tapia
    Department of Immunology, Laboratory of Molecular Immunology II, National School of Biological Sciences, Mexico City, Mexico
  • M. C. Jimenez-Martinez
    Department of Immunology/Research Unit, Institute of Ophthalmology, Mexico City, Mexico
    Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
  • Footnotes
    Commercial Relationships  S. Groman-Lupa, None; L. Vizuet-Garcia, None; G.A. Luna-Baca, None; A. Robles-Contreras, None; E. Rivera-Mendez, None; J. Serafin, None; I. Estrada-Garcia, None; S. Estrada-Parra S, None; M. Perez-Tapia, None; M.C. Jimenez-Martinez, None.
  • Footnotes
    Support  CONACyT grant 71291
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 415. doi:
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      S. Groman-Lupa, L. Vizuet-Garcia, G. A. Luna-Baca, A. Robles-Contreras, E. Rivera-Mendez, J. Serafin, I. Estrada-Garcia, S. Estrada-Parra S, M. Perez-Tapia, M. C. Jimenez-Martinez; Dyalizable Leukocyte Extracts and TLR Agonist Effects on VEGF Secretion by Human Limbal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):415.

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Abstract

Purpose: : To study the effect of Dializable Leukocyte Extracts (DLE) and TLR agonists on VEGF secretion by Human Limbal Epithelial Cells (HuLEC).

Methods: : HuLEC were obtained from cadaveric corneal-scleral rings and cultured with KSFM until confluence. Then, HuLEC were stimulated at optimal concentrations with specific TLR1-9 agonists (TLRa): Pam3CSK4, HKLM, Poly I:C, LPS, Flagellin, FSL-1, Imiquimod, ssRNA40 and ODN2006, PMA/Ionomicyne or DLE. Supernatants were collected at different times and analyzed to determine VEGF concentrations by ELISA.

Results: : VEGF concentrations at 24h: Non Treated HuLEC 315.1pg/ml, PMA/Ionomicyne 395pg/ml; Pam3CSK4 treated HuLEC 262.2pg/mL, HKLM treated HuLEC 415.8pg/mL, Poly I:C treated HuLEC, 430.3pg/mL, LPS treated HuLEC 307.2pg/mL, Flagellin treated HuLEC 340.0pg/mL, FSL-1 treated cells 343.6pg/mL, Imiquimod treated HulEC 433.6pg/mL, ssRNA40 treated cells 355.0pg/mL, ODN2006 treated cells 322.4pg/mL, DLE 0.5µg/mL treated HuLEC 755.4pg/mL, DLE 5µg/mL treated HuLEC 1310pg/mL.

Conclusions: : DLE induced up-regulation of VEGF secretion by HuLEC. These results suggest that DLE stimulation could be used as an in vitro model to study molecules related to neovascularization in cells derived from ocular surface. Stimulation trough TLRa (HKLM, Poly I:C and Imiquimod) could be associated with innate pathways of neovascularization in HuLEC. Additional studies are needed to assess this question.

Keywords: vascular endothelial growth factor • neovascularization • cornea: basic science 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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