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T. Ecoiffier, D. Yuen, L. Chen; Nasal Dominant Distribution of the Blood and Lymphatic Vessels in the Cornea. Invest. Ophthalmol. Vis. Sci. 2010;51(13):417.
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Due to its unique characteristics, the cornea has been widely used for blood and lymphatic vessel research. However, it has never been studied whether limbal or corneal vessels are evenly distributed under normal or inflamed conditions. The purpose of this study was to investigate this question and to examine whether and how the distribution patterns change during corneal inflammatory lymphangiogenesis (LG) and hemangiogenesis (HG).
Corneal inflammatory LG and HG was induced in two most commonly used mouse strains, BALB/c and C57BL/6 (6-8 weeks of age), by a standardized two-suture placement model. Oriented flat-mount corneas together with the limbal tissues were used for immunofluorescent microscopic studies. Blood and lymphatic vessels under both normal and inflamed conditions were analyzed and quantified by NIH Image J software to compare their distributions.
The data demonstrate, for the first time, that both blood and lymphatic vessels were more distributed in the nasal side in normal murine limbal areas. This nasal dominant pattern was maintained during corneal inflammatory LG while it was lost for HG.
Blood and lymphatic vessels are not evenly distributed in normal limbal areas. Furthermore, corneal LG and HG respond to inflammatory stimulus in different manners. These new findings will shed some light on our understanding of corneal physiology and pathogenesis and the development of experimental models and therapeutic strategies for corneal diseases.
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