April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Lipofuscin Distribution in Retinal Pigment Epithelium of Rhesus Monkeys: Effects of Age, n-3 Fatty Acids, and Xanthophylls
Author Affiliations & Notes
  • M. Snodderly
    Nutritional Sci, University of Texas at Austin, Austin, Texas
  • I. Y. Leung
    Wilmer Eye Institute, Johns Hopkins University, Maryland
  • M. Neuringer
    ONPRC, Beaverton, Oregon
    Casey Eye Institute, Oregon Health and Science University, Oregon
  • Footnotes
    Commercial Relationships  M. Snodderly, DSM Nutritional Products Ltd, F; I.Y. Leung, DSM Nutritional Products Ltd, F; M. Neuringer, DSM Nutritional Products Ltd, F.
  • Footnotes
    Support  DSM Nutritional Products Ltd., NIH P30 EY03790, NIH DK-29930, NIH RR-00163, The Foundation Fighting Blindness, John Linn Foundation, Dennis Gierhart Foundation
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 472. doi:
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      M. Snodderly, I. Y. Leung, M. Neuringer; Lipofuscin Distribution in Retinal Pigment Epithelium of Rhesus Monkeys: Effects of Age, n-3 Fatty Acids, and Xanthophylls. Invest. Ophthalmol. Vis. Sci. 2010;51(13):472.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To study the effects of n-3 fatty acids, lutein and zeaxanthin on the distribution of lipofuscin in the retinal pigment epithelium (RPE).

Methods: : Seventeen rhesus monkeys, 7-18 years of age, were fed xanthophyll-free semipurified diets with either low or adequate amount of n-3 fatty acids from birth and had no macular pigment. Five were supplemented with lutein and six with zeaxanthin for 6 to 24 months while six remained on the xanthophyll-free diet. Following sacrifice, the central retina was sectioned at 2 µm along the vertical meridian through the fovea. Fluorescent images of the central retinal sections were taken with Texas Red and/or FITC filter sets. The areas occupied by the autofluorescent lipofuscin granules of the RPE were measured along the vertical meridian from zero to 2.64 mm eccentricity and compared with data from age-matched control monkeys (n = 7) fed a standard laboratory diet.

Results: : In animals fed the xanthophyll-free diet low in n-3 fatty acids, the RPE accumulated more lipofuscin and at a faster rate with advancing age than control animals (p 0.1).

Conclusions: : Long-term dietary deprivation of n-3 fatty acids, in the absence of lutein and zeaxanthin, increased the accumulation of lipofuscin in the parafoveal RPE. Shorter-term supplementation with zeaxanthin or lutein did not reverse this effect. Reduction in lipofuscin levels is one mechanism by which n-3 fatty acids may confer protection from age-related macular disease.

Keywords: ipofuscin • macular pigment • lipids 

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