April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Regulatory Effect of Vascular Endothelial Cell PD-L1 on Angiogenesis
Author Affiliations & Notes
  • Y. Jin
    Schepens Eye Research, Boston, Massachusetts
  • S. K. Chauhan
    Schepens Eye Research, Boston, Massachusetts
  • P. Sage
    Department of Pathology, Harvard Medical School, Boston, Massachusetts
  • A. Sharpe
    Department of Pathology, Harvard Medical School, Boston, Massachusetts
  • R. Dana
    Schepens Eye Research, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  Y. Jin, None; S.K. Chauhan, None; P. Sage, None; A. Sharpe, None; R. Dana, None.
  • Footnotes
    Support  NEI-12963
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 49. doi:
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      Y. Jin, S. K. Chauhan, P. Sage, A. Sharpe, R. Dana; Regulatory Effect of Vascular Endothelial Cell PD-L1 on Angiogenesis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):49.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigate the role of program death ligand-1 (PD-L1) expression on vascular endothelial cell (VEC) proliferation and corneal angiogenesis.

Methods: : Expression levels of PD-L1, PD-1 and CD80 (B7.1) in MS1 cells (mouse VEC cell line) and primary VEC from murine lung and heart were analyzed using real-time PCR and flow cytometry. PD-L1 and CD80 expression levels in the MS1 cells were inhibited using respective siRNA, and the cell proliferation was then analyzed by BrdU incorporation assay. Neovascularization in the PD-L1 knockout (KO) mice and wild-type (WT) mice were scored during 2 weeks after suture-placement in the cornea using slit-lamp biomicroscopy, and the percentage of CD31hiLYVE-1- blood vessels in the corneas were measured on Day 14 using confocal microscopy. The expression levels of VEGF-A and VEGFR2 on the MS1 cells or the suture-placed corneas were analyzed by real-time PCR.

Results: : Both primary murine VEC and MS1 cells expressed PD-L1 and CD80, but not PD-1, at mRNA and protein levels. After inhibition of PD-L1 or CD80 expression in the MS1 cells by siRNA transfection, the mRNA expression level of VEGFR2 and the cell proliferation level were significantly enhanced, compared to the control group (p<0.01, t-test). After suture placement in the cornea, the expression level of VEGFR2 was significantly higher in the PD-L1 KO mice vs. WT mice (p<0.05, t-test). The CD31hiLYVE-1- blood vessel density was significantly promoted in the PDL-1KO group relative to the WT group (22+2% vs.11+1%, n=6 per group, p<0.001, t-test).

Conclusions: : The expression of PD-L1 and its receptor CD80 on VEC has an inhibitory function on VEC proliferation and angiogenesis.

Keywords: neovascularization • cornea: basic science • vascular endothelial growth factor 

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