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G. Gokce, T. Ozgurtas, G. Sobaci; The Effects of Amphotericin B and Voriconazole on in vivo Angiogenesis in Chick Chorioallantoic Membrane. Invest. Ophthalmol. Vis. Sci. 2010;51(13):55.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the in-vivo anti-angiogenic effect of Amphotericin B and Voriconazole which are commonly used in the treatment of fungal endophthalmitis either systemically or intravitreally in chick chorioallantoic membrane (CAM) model.
Atak-S type fertilized eggs obtained from Poultry Institution (Ankara, TURKIYE) were used. The eggs were kept under 37o C at 85-90 % relative humidity until fifth day when the application would be performed. Amphotericin B and Voriconazole were applied to eggs at different concentrations (Amphotericin B 125ng, 125µg; Voriconazole 100ng, 100µg). Thirteen eggs were used for each group. The eggs were kept under appropriate condition throughout the experiment. The results were evaluated at the 48th hour of application of the drugs and recorded by digital camera (Konica Minolta, Tokyo, JAPAN).
No anti-angiogenic effect was observed in the eggs treated with all concentrations of voriconazole and all the eggs treated with 125 ng Amphotericin B. A significant anti-angiogenic effect was determined in 125 µg Amphotericin B group (11 of 13 eggs, 84.6 %).
Anti-angiogenic effect seen in therapeutic doses of Amphotericin B in this model may indicate potential use of this drug as an anti-angiogenic agent.
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