April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Hypoxia-Induecd Insulin-Like Growth Factor-2 Regulates Expression of Vascular Endothelial Growth Factor in Retinal Endothelial Cells
Author Affiliations & Notes
  • S. Park
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Kim
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • K.-W. Kim
    NeuroVascular Coordination Research Center, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea
  • Y. Yu
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Kim
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  S. Park, None; J. Kim, None; K.-W. Kim, None; Y. Yu, None; J. Kim, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 57. doi:
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      S. Park, J. Kim, K.-W. Kim, Y. Yu, J. Kim; Hypoxia-Induecd Insulin-Like Growth Factor-2 Regulates Expression of Vascular Endothelial Growth Factor in Retinal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):57.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the role of insulin-like growth factor (IGF)-2 in vascular endothelial growth factor (VEGF) expression in retinal endothelial cells under hypoxia.

Methods: : In human retinal microvascular endothelial cells, IGF-2 and VEGF expression were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis under hypoxic condition (< 1%). To investigate IGF-2 induced regulation of VEGF expression in HRMEC, western blot analysis for VEGF, ERK-1/2, p-ERK-1/2, Akt, and p-Akt was performed with treatment of IGF-2 (1 to 1000 ng/ml) In addition, VEGF expression in HRMEC was evaluated under hypoxia with treatment of a blocking antibody to IGF-2 by RT-PCR and Western blot analysis.

Results: : Under hypoxic condition, IGF-2 and VEGF were up-regulated in HRMEC. VEGF expression increased in dose-dependent manner with treatment of IGF-2, which was accompanied by the significant increase of ERK as well as Akt phosphorylation. Moreover, hypoxia-induced VEGF expression was effectively inhibited by blocking of IGF-2.

Conclusions: : Our results suggest that hypoxia-induced IGF-2 could directly regulate VEGF expression in HRMEC via ERK as well as PI3K/Akt pathway. Therefore, IGF-2 might be involved in regulation of developing retinal vessels which is mediated by hypoxia-induced VEGF expression.

Keywords: growth factors/growth factor receptors • vascular endothelial growth factor • hypoxia 
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