Purchase this article with an account.
D. Izhaky, A. S. Vilupuru, J. A. Burke, D. A. Nelson, A. Grinvald; Assaying Recovery of Retinal Metabolism Following Photic Challenge in Normal and Pathological Monkey Retina. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1061.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Retinal NIR reflectance changes in response to photic stimulation carry information about metabolic processes following the retina photic activation. The Retinal Function Imager (RFI, Optical Imaging, Ltd.) was used to non-invasively assess the recovery of such metabolic signals following strong retina photic activation in anesthetized monkeys which had retinal pathologies.
The RFI measures activity-dependent functional metabolic signals in retinal image series recorded at chosen wavelengths. The monkeys’ eyes were exposed to bright light (548 nm) for 5 minutes followed by functional metabolic NIR imaging (750 nm) in the dark every minute for 20-30 minutes. Each imaging trial lasted for 10 seconds with a 1 second green circular grid pattern projected on the retina 2 seconds into the trial.The relative evoked functional response to the pattern stimulus was evaluated to assess the recovery of the standards metabolic signal over time.
The functional signal was absent following exposure to light and gradually recovered in the dark. The recovery took 12-18 minutes for normal retina. Delayed recovery time and reduced amplitude of the metabolic signals were observed in monkeys with medium and severe ocular hypertension (OHT) and monkeys with branched retinal vein occlusion (BRVO).
Prolonged recovery time due to glaucomatous or BRVO damage was detected non-invasively using functional intrinsic metabolic imaging. It is likely that the same functional assay may prove useful to evaluate other retinal pathologies as well as an assay for drug development.
This PDF is available to Subscribers Only