April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Assaying Recovery of Retinal Metabolism Following Photic Challenge in Normal and Pathological Monkey Retina
Author Affiliations & Notes
  • D. Izhaky
    Optical Imaging Ltd, Rehovot, Israel
  • A. S. Vilupuru
    Biological Sciences, Allergan, Inc, Irvine, California
  • J. A. Burke
    Biological Sciences, Allergan, Inc, Irvine, California
  • D. A. Nelson
    Optical Imaging Ltd, Rehovot, Israel
  • A. Grinvald
    Neurobiology, Weizmann Inst of Science, Rehovot, Israel
  • Footnotes
    Commercial Relationships  D. Izhaky, Optical Imaging Ltd, E; A.S. Vilupuru, Allergan Inc., E; J.A. Burke, Allergan Inc., E; D.A. Nelson, Optical Imaging Ltd, E; Optical Imaging Ltd, P; A. Grinvald, Optical Imaging Ltd, I; Optical Imaging Ltd, P.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1061. doi:
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      D. Izhaky, A. S. Vilupuru, J. A. Burke, D. A. Nelson, A. Grinvald; Assaying Recovery of Retinal Metabolism Following Photic Challenge in Normal and Pathological Monkey Retina. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1061.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Retinal NIR reflectance changes in response to photic stimulation carry information about metabolic processes following the retina photic activation. The Retinal Function Imager (RFI, Optical Imaging, Ltd.) was used to non-invasively assess the recovery of such metabolic signals following strong retina photic activation in anesthetized monkeys which had retinal pathologies.

Methods: : The RFI measures activity-dependent functional metabolic signals in retinal image series recorded at chosen wavelengths. The monkeys’ eyes were exposed to bright light (548 nm) for 5 minutes followed by functional metabolic NIR imaging (750 nm) in the dark every minute for 20-30 minutes. Each imaging trial lasted for 10 seconds with a 1 second green circular grid pattern projected on the retina 2 seconds into the trial.The relative evoked functional response to the pattern stimulus was evaluated to assess the recovery of the standards metabolic signal over time.

Results: : The functional signal was absent following exposure to light and gradually recovered in the dark. The recovery took 12-18 minutes for normal retina. Delayed recovery time and reduced amplitude of the metabolic signals were observed in monkeys with medium and severe ocular hypertension (OHT) and monkeys with branched retinal vein occlusion (BRVO).

Conclusions: : Prolonged recovery time due to glaucomatous or BRVO damage was detected non-invasively using functional intrinsic metabolic imaging. It is likely that the same functional assay may prove useful to evaluate other retinal pathologies as well as an assay for drug development.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • retina: proximal (bipolar, amacrine, and ganglion cells) • metabolism 
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