April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Role of Ca2+-Dependent Currents in the Recovery Phase of Rod ERG Dim-Flash Responses
Author Affiliations & Notes
  • F. Vinberg
    BECS, Aalto University, Espoo, Finland
  • A. Koskelainen
    BECS, Aalto University, Espoo, Finland
  • Footnotes
    Commercial Relationships  F. Vinberg, None; A. Koskelainen, None.
  • Footnotes
    Support  Finnish Academy grant #128081 and 111866
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1071. doi:
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      F. Vinberg, A. Koskelainen; Role of Ca2+-Dependent Currents in the Recovery Phase of Rod ERG Dim-Flash Responses. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1071.

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Abstract

Purpose: : The electroretinogram (ERG) is a field potential that is generated by several current dipoles (source/sink pairs) within the retina. Our previous studies have suggested that the fast negative peak ("nose"1) in saturated mouse ERG photoresponses is generated by voltage-dependent h- and Kx channels in rod inner segment2,3. In this study we investigate the current sources/sinks that might affect the recovery phase of the mouse rod dim-flash ERG responses.

Methods: : Photoresponses to flashes (2 - 20 ms) were recorded with the ERG technique across isolated mouse (Mus musculus) retinas. The b-wave and higher-order neuron components were blocked by 2 mM aspartate or 0.05 mM APB. The glial component (slow PIII) was removed by BaCl2.

Results: : Rod responses to dim flashes sometimes include a secondary negative wave. This wave was always present and greatly amplified when the rods were loaded with the intracellular Ca2+ buffer BAPTA(-AM). The secondary wave of dim-flash responses present in BAPTA-loaded rods could be abolished by the Ca2+ channel blocker Cd2+ (0.1 mM).

Conclusions: : The removal of the secondary wave of dim-flash responses by Cd2+ suggests that the Ca2+-activated channels (K+ or Cl- channels) may affect the recovery phase of the ERG signal.1Arden G.B. (1976), J. Physiol. 256(2):333-3602Vinberg F. & Koskelainen A. (2009), Invest. Ophthalmol. Vis. Sci. 50: E- Abstract 21753Vinberg F., Strandman S. & Koskelainen (2009), J. Vis. 9(12):9.1-17

Keywords: electroretinography: non-clinical • calcium • ion channels 
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