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C. K. Vorwerk, C. Mawrin; Retinal Activation of the Creb-Dependent Genes C-fos and Bcl-2 Following Acute Optic Nerve Damage. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1081.
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To determine the role of mitogen-activated protein kinase (MAPK)-dependent cAMP response element binding protein (CREB) activation on c-Fos and Bcl-2 gene expression in animal model of acute optic nerve damage.
An rat model of acute optic nerve crush was used to study the time course of pMAPK, pAkt, pCREB, c-Fos and bcl-2 expression by immunohistochemistry, western blotting, and real-time PCR. Ganglion cell death was evaluated by Fluoro-Jade and TUNEL staining.
Evaluation of the time course in acute optic nerve damage revealed an activation of pMAPK and pCREB but a depression of pAkt expression. Real-time PCR showed that the pMAPK-pCREB activation is associated with increased c-Fos mRNA but reduced Bcl-2 mRNA levels.
Retinal ganglion cell death in the early phase after acute optic nerve trauma is due to an imbalance of pro- and anti-apoptotic signal cascades. With progressing time neuroprotective, anti-apoptotic factors, such as BCL-2 are activated to minimize secondary injury.
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