April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Long-Term Results of Autologous Cultivated Corneal Limbal Epithelial Transplantation in Severe Ocular Surface Disorders
Author Affiliations & Notes
  • T. Nakamura
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-ku, Japan
    Research Center for Inflammation and Regenerative Medicine, Karasuma Imadegawa Kamigyo-ku Kyoto, Japan
  • T. Inatomi
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-ku, Japan
  • C. Sotozono
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • N. Koizumi
    Biomedical Engineering, Doshisha University, Kyotanabe city, Japan
  • S. Kinoshita
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-Ku, Japan
  • Footnotes
    Commercial Relationships  T. Nakamura, None; T. Inatomi, None; C. Sotozono, None; N. Koizumi, None; S. Kinoshita, None.
  • Footnotes
    Support  H16–Saisei-007
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1136. doi:
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      T. Nakamura, T. Inatomi, C. Sotozono, N. Koizumi, S. Kinoshita; Long-Term Results of Autologous Cultivated Corneal Limbal Epithelial Transplantation in Severe Ocular Surface Disorders. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1136.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigate the long-term outcome of autologous cultivated corneal limbal epithelial transplantation (CLET) for the treatment of unilateral severe ocular surface disorders (OSD).

Methods: : This study involved 6 eyes of 6 patients who received autologous CLET for total limbal stem-cell deficiency. These consisted of 3 eyes with thermal and chemical injury and 3 eyes with idiopathic OSD. Autologous CLET sheets were created using amniotic membrane as a substrate. Clinical efficacy was evaluated by the postoperative visual acuity (VA). The clinical results (clinical conjunctivalization, corneal neovascularization, and symblepharon formation) were evaluated and graded on a scale from 0 to 3 according to their severity. Clinical safety was evaluated by persistent epithelial defects, ocular hypertension, and infections.

Results: : Autologous CLET sheets were successfully generated from all 6 patients. The patients were followed-up for a mean period of 48 months; the longest follow-up period was 80 months. Best corrected VA and final VA was improved in 5 eyes (83.3%). During the long-term follow-up period, postoperative complications such as persistent epithelial defects, ocular hypertension and symblepharon formation were not observed. All cases showed a various degree of mild superficial clinical conjunctivalization extending from the limbal region.

Conclusions: : These long-term clinical results strongly support the conclusion that tissue-engineered CLET sheets are useful for reconstructing the ocular surface of unilateral severe OSD.

Clinical Trial: : 61396

Keywords: cornea: clinical science • cornea: epithelium 

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