April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Covalent Attachment of N,N-Dodecyl,Methyl-Polyethylenimine to Boston Keratoprosthesis Materials Inhibits S. aureus Biofilm Formation Without Cytotoxicity or Corneal Cell Reactivity
Author Affiliations & Notes
  • I. Behlau
    Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
    Ophthalmology, Schepens Eye Research Institute, Boston, Massachusetts
  • K. Mukherjee
    Chemistry and Bioengineering, Massachusetts Institute of Technology, Cambridge, Massachusetts
  • A. Todani
    Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
  • A. M. Klibanov
    Chemistry and Bioengineering, Massachusetts Institute of Technology, Cambridge, Massachusetts
  • M. S. Gilmore
    Ophthalmology, Schepens Eye Research Institute, Boston, Massachusetts
  • S. J. Spurr-Michaud
    Ophthalmology, Schepens Eye Research Institute, Boston, Massachusetts
  • A. S. Tisdale
    Ophthalmology, Schepens Eye Research Institute, Boston, Massachusetts
  • F. Cade
    Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
  • C. H. Dohlman
    Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  I. Behlau, None; K. Mukherjee, None; A. Todani, None; A.M. Klibanov, None; M.S. Gilmore, None; S.J. Spurr-Michaud, None; A.S. Tisdale, None; F. Cade, None; C.H. Dohlman, None.
  • Footnotes
    Support  Fight for Sight (I.B.); The Kpro Fund
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1148. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      I. Behlau, K. Mukherjee, A. Todani, A. M. Klibanov, M. S. Gilmore, S. J. Spurr-Michaud, A. S. Tisdale, F. Cade, C. H. Dohlman; Covalent Attachment of N,N-Dodecyl,Methyl-Polyethylenimine to Boston Keratoprosthesis Materials Inhibits S. aureus Biofilm Formation Without Cytotoxicity or Corneal Cell Reactivity. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1148.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To minimize bacterial adherence and biofilm formation by comparative analysis of new polycations bound to bio-prosthetic ocular-associated materials, particularly poly (methyl methacrylate) (PMMA), using the Boston Keratoprosthesis as a model system.

Methods: : Quantitative assessments of S. aureus microbial biofilm formation by a linear version of N, N-dodecyl, methyl-polyethyleneimine (DMPEI) (217 kDa) covalently bound to PMMA (DMPEI-PMMA) compared to PMMA respectively was performed. DMPEI-bound materials compared to the unbound original materials were screened for corneal reactivity in both cell tissue culture and in vivo in the rabbit. By using a newly developed intrastromal infection model in the rodent, the antimicrobial efficacy of DMPEI-PMMA compared to parent PMMA is being determined.

Results: : There is an inhibitory effect on S. aureus biofilm formation by DMPEI-derivatized materials compared to the parent PMMA. DMPEI-PMMA did not confer any additional epithelial cell cytotoxicity in vitro nor corneal reactivity in vivo in the rabbit. Assessment of the antimicrobial efficacy of DMPEI-PMMA in an intrastromal infection model in rodent species is ongoing.

Conclusions: : We found that covalent derivatization with DMPEI of PMMA greatly reduces S. aureus biofilm formation compared to the parent materials in vitro. There is no additional cytotoxicity seen in either a human corneal epithelial cell tissue culture model or an intrastromal rabbit model in vivo. Ongoing studies are evaluating DMPEI-derivatized materials for in vivo antimicrobial efficacy in a new intrastromal infection model in rodent species.

Keywords: Staphylococcus • keratoprostheses • microbial pathogenesis: experimental studies 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×