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I. Behlau, K. Mukherjee, A. Todani, A. M. Klibanov, M. S. Gilmore, S. J. Spurr-Michaud, A. S. Tisdale, F. Cade, C. H. Dohlman; Covalent Attachment of N,N-Dodecyl,Methyl-Polyethylenimine to Boston Keratoprosthesis Materials Inhibits S. aureus Biofilm Formation Without Cytotoxicity or Corneal Cell Reactivity. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1148.
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To minimize bacterial adherence and biofilm formation by comparative analysis of new polycations bound to bio-prosthetic ocular-associated materials, particularly poly (methyl methacrylate) (PMMA), using the Boston Keratoprosthesis as a model system.
Quantitative assessments of S. aureus microbial biofilm formation by a linear version of N, N-dodecyl, methyl-polyethyleneimine (DMPEI) (217 kDa) covalently bound to PMMA (DMPEI-PMMA) compared to PMMA respectively was performed. DMPEI-bound materials compared to the unbound original materials were screened for corneal reactivity in both cell tissue culture and in vivo in the rabbit. By using a newly developed intrastromal infection model in the rodent, the antimicrobial efficacy of DMPEI-PMMA compared to parent PMMA is being determined.
There is an inhibitory effect on S. aureus biofilm formation by DMPEI-derivatized materials compared to the parent PMMA. DMPEI-PMMA did not confer any additional epithelial cell cytotoxicity in vitro nor corneal reactivity in vivo in the rabbit. Assessment of the antimicrobial efficacy of DMPEI-PMMA in an intrastromal infection model in rodent species is ongoing.
We found that covalent derivatization with DMPEI of PMMA greatly reduces S. aureus biofilm formation compared to the parent materials in vitro. There is no additional cytotoxicity seen in either a human corneal epithelial cell tissue culture model or an intrastromal rabbit model in vivo. Ongoing studies are evaluating DMPEI-derivatized materials for in vivo antimicrobial efficacy in a new intrastromal infection model in rodent species.
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