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S. Lofgren, P. Andertun; Time-Dependent Change in Glutathione Peroxidase Activity in H2O2-Treated Lenses From Wild-Type and Thioltransferase/glutaredoxin-1 Knockout Mice. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1179.
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© ARVO (1962-2015); The Authors (2016-present)
To study oxidative stress response in cultured lenses.
Lenses from wild-type and thioltransferase/glutaredoxin-1 knockout mice were incubated with 0.5 mM H2O2. The lenses were homogenized at day 1, 2, and 4 and the activity of glutathione-related enzymes were quantified photometrically. Protein was quantified with the BCA assay.
The activity of glutathione peroxidase decreased significantly the first two days, and normalized at day 4, with indication of increased activity in the wild-type lenses. The activity at day 1 and 2 was significantly lower than at day 4. There was no significant difference between the wild-type and the knockout lenses. Other planned enzyme measurements were not finished at the time of the abstract submission.
Glutathione peroxidase activity decreases initially after oxidative stress and recovers later on. This pattern varies with species, tissue and oxidant.
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