Abstract
Purpose: :
To study oxidative stress response in cultured lenses.
Methods: :
Lenses from wild-type and thioltransferase/glutaredoxin-1 knockout mice were incubated with 0.5 mM H2O2. The lenses were homogenized at day 1, 2, and 4 and the activity of glutathione-related enzymes were quantified photometrically. Protein was quantified with the BCA assay.
Results: :
The activity of glutathione peroxidase decreased significantly the first two days, and normalized at day 4, with indication of increased activity in the wild-type lenses. The activity at day 1 and 2 was significantly lower than at day 4. There was no significant difference between the wild-type and the knockout lenses. Other planned enzyme measurements were not finished at the time of the abstract submission.
Conclusions: :
Glutathione peroxidase activity decreases initially after oxidative stress and recovers later on. This pattern varies with species, tissue and oxidant.
Keywords: oxidation/oxidative or free radical damage