April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Evolution of p53 and Caspase 3 Expression in the Rat Lens After in vivo Exposure to UV-B Radiation
Author Affiliations & Notes
  • K. Galichanin
    GullstrandLab, Section of Ophthalmology,
    Uppsala University, Uppsala, Sweden
    Karolinska Institutet, St. Eriks Eye Hospital, Stockholm, Sweden
  • J. Svedlund
    Department of Surgical Sciences,
    Uppsala University, Uppsala, Sweden
  • S. Löfgren
    Karolinska Institutet, St. Eriks Eye Hospital, Stockholm, Sweden
  • P. G. Söderberg
    GullstrandLab, Section of Ophthalmology,
    Uppsala University, Uppsala, Sweden
  • Footnotes
    Commercial Relationships  K. Galichanin, None; J. Svedlund, None; S. Löfgren, None; P.G. Söderberg, None.
  • Footnotes
    Support  Karolinska Institute Eye Research Foundation, Gun och Bertil Stohnes Stiftelse, Karin Sandqvists Stiftelse, St. Eriks Eye Research Foundation, Swedish Research Council
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1186. doi:
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    • Get Citation

      K. Galichanin, J. Svedlund, S. Löfgren, P. G. Söderberg; Evolution of p53 and Caspase 3 Expression in the Rat Lens After in vivo Exposure to UV-B Radiation. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1186.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate evolution of p53 and caspase 3 mRNA expression ratios between exposed lenses and their contralateral nonexposed controls after in vivo close to threshold ultraviolet-B radiation (UVR-B) around 300 nm.

Methods: : Forty 6-week-old female albino Sprague-Dawley rats received double threshold dose (8 kJ/m2) UVR (max = 300 nm) unilaterally for 15 minutes. The animals were sacrificed at 1, 5, 24 and 120 h following exposure, depending on the group belonging. p53 and caspase 3 expression of the lenses was analysed by quantitative RT-PCR. Data were analysed using one-way ANOVA.

Results: : p53 is downregulated in exposed lenses in 1 h and 5 h groups and upregulated in 24 h and 120 h groups. Caspase 3 is downregulated in exposed lenses in 1, 5 and 24 h groups and upregulated in 120 h group.

Conclusions: : Apoptosis in the rat lens is a dynamic process after in vivo exposure to UVR-B. p53 mRNA increased expression precedes caspase 3 mRNA increased expression in the rat lens after in vivo UVR-B exposure.

Keywords: cataract • cell survival • radiation damage: light/UV 
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