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L. E. Goldstein, C. J. Rosen, K. A. Bjornstad, J. A. Moncaster, N. F. Casey, A. C. Thompson, A. Fraine, O. Kempf, P. Y. Chang, E. A. Blakely; Non-Invasive Quasi-Elastic Light Scattering (QLS) Assessment of the Pre-Cataractous Lens for Space Radiation Biodosimetry in Mice. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1190.
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© ARVO (1962-2015); The Authors (2016-present)
The lens is a highly ordered tissue with unique optical properties and exquisite radiosensitivity. The goal of this project is to investigate the natural history of Rayleigh light scattering changes in pre-cataractous lenses of mice exposed to high-energy proton or iron particle radiations prevalent in space. A secondary goal was to assess the efficacy of a purpose-designed quasi-elastic light scattering (QLS) instrument as a non-invasive molecular biodosimetry platform for detecting and monitoring exposure to biologically relevant space radiation.
C57Bl6 male mice (25 males/group) were irradiated with 10 or 100 cGy of 1.0 GeV/amu protons (LET~0.22 kev/µm) or 1 GeV/amu iron ions (LET~155 keV/µm) at the NASA Space Radiation, Brookhaven National Laboratory (BNL). Control mice (n=10 males) were sham irradiated. For each examination, the left eye was dilated (1% tropicamide) and the lens assessed by QLS without anesthesia. Each examination consists of 15 autocorrelation acquisitions with infrared slit-lamp imaging of the sampled lens region. Autocorrelation and scattering intensity analyses were conducted on data averaged over each 15-acquisition test session and compared to conventional slit lamp biomicroscopy conducted on the same mice. Baseline QLS was conducted on all mice prior to irradiation. Polystyrene bead (0.2, 0.1, 0.05 µm) were used for QLS instrument calibration.
We demonstrated intra-assay precision of the QLS platform and successfully acquired longitudinal autocorrelation function and Raleigh scattering intensity values from non-anesthetized mice in irradiated and sham cohorts. QLS and slit-lamp examinations were compared.
Non-invasive QLS instrumentation for in vivo lens assessment has been succesfully deployed to study the longitudinal effects of space radiation exposure in mice.
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