April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Experimental Model of Deep Anterior Lamellar Keratoplasty (DALK) With Desiccated Cornea in Rabbits
Author Affiliations & Notes
  • G. F. Cunha
    Ocular Surface and Refractive Surgery,
    Fundacion de Investigacion Oftalmologica, Oviedo, Spain
  • I. Alcalde
    Ocular Surface and Refractive Surgery,
    Fundacion de Investigacion Oftalmologica, Oviedo, Spain
  • S. del Olmo-Aguado
    Ocular Surface and Refractive Surgery,
    Fundacion de Investigacion Oftalmologica, Oviedo, Spain
  • M. Chacon
    Instituto Oftalmologico Fernandez-Vega,
    Fundacion de Investigacion Oftalmologica, Oviedo, Spain
  • C. Alvarez
    Instituto Oftalmologico Fernandez-Vega,
    Fundacion de Investigacion Oftalmologica, Oviedo, Spain
  • J. Merayo-Lloves
    Ocular Surface and Refractive Surgery,
    Fundacion de Investigacion Oftalmologica, Oviedo, Spain
  • Footnotes
    Commercial Relationships  G.F. Cunha, None; I. Alcalde, None; S. del Olmo-Aguado, None; M. Chacon, None; C. Alvarez, None; J. Merayo-Lloves, None.
  • Footnotes
    Support  Supported by FIO
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 734. doi:
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    • Get Citation

      G. F. Cunha, I. Alcalde, S. del Olmo-Aguado, M. Chacon, C. Alvarez, J. Merayo-Lloves; Experimental Model of Deep Anterior Lamellar Keratoplasty (DALK) With Desiccated Cornea in Rabbits. Invest. Ophthalmol. Vis. Sci. 2010;51(13):734.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The aim of this work is to assess the viability of dehydrated cornea to lamellar keratoplasty.

Methods: : Rabbit corneas were desiccated for 30 days in a vacuum desiccation chamber before the Deep Anterior Lamellar Keratoplasty (DALK) procedure. After re-hydration the cornea for 30 minutes in sterile irrigating solution (BSS), surgeries were performed following a Ferrara’s technique with a modified Ferrara’s spatula and a 6.0 monofilament suture. Rabbits were sacrificed at day 7, 15, 30 and 90 following the lamellar keratoplasty. Outcomes were measured by clinical signs, cell biology (Hematoxylin - eosin, Periodic acid of Schiff and Masson trichrome) and immunohistochemistry (Thy1.1, α-SMA, BrdU, TUJ1).

Results: : All animals developed mild conjunctival injection and stromal haze 24 hours postop. No conjunctival injection was detected on evaluation at day 7 with more corneal transparency. Light microscopy demonstrated monolayer of epithelial cells and a stromal infiltration of inflammatory cells with activated keratocytes (α-SMA positive). On day 15 and 30, a multilayer of epithelial cells was present in all corneal surface. Regarding stroma, inflammatory cells were reduced and activated keratocytes and newly formed nerve fibers were present.

Conclusions: : Dehydrated cornea could be used for DALK procedures with good integration and viability in the recipient tissue.

Keywords: cornea: basic science • regeneration • wound healing 
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