April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Twelve Months Results of Xenograft in Descemet’s Membrane Endothelial Keratoplasty Using Synthetic Carrier and Injector System: Experimental Animal Eye Model
Author Affiliations & Notes
  • S. Chang
    Ophthalmology, Keimyung University Sch of Med, Daegu, Republic of Korea
    Cornea and anterior segment service, Wilmer Eye institute, Johns Hopkins University, Baltimore, Maryland
  • J. Lee
    Ophthalmology, Keimyung University Sch of Med, Daegu, Republic of Korea
  • J. Lee
    Ophthalmology, Keimyung University Sch of Med, Daegu, Republic of Korea
  • Footnotes
    Commercial Relationships  S. Chang, None; J. Lee, None; J. Lee, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 747. doi:
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      S. Chang, J. Lee, J. Lee; Twelve Months Results of Xenograft in Descemet’s Membrane Endothelial Keratoplasty Using Synthetic Carrier and Injector System: Experimental Animal Eye Model. Invest. Ophthalmol. Vis. Sci. 2010;51(13):747.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The authors report the twelve months results of xenografts in Descemet Membrane Endothelial Keratoplasty using synthetic carrier and injector system: In vivo experimental animal study.

Methods: : Six Korean native mixed species cats and six eyes of three rabbits were used for the experiments. Cats were used as recipients and rabbits were used as donor. Descemetorhexis was made at the recipient’s eye. Descemet-endothelium complexes were obtained through suprachoroidal approach (2008 ARVO poster #1961). Harvested Descemet’s membrane-endothelium complexes were carried on synthetic carrier (soft contact lens). After the two punches of steps down, Descemet’s membrane-endothelium-carrier complexes were loaded on injector system. The complexes were injected into the anterior chamber and attached behind the stroma. Slit lamp biomicroscopic examination, pachymetry and endothelial cell densities were evaluated.

Results: : Success rate of transplantation was 83.3 % (5/6). One case was failed due to the flap detachment. Corneal thickness was markedly increased (135%, p=0.072, anova test) at 2 weeks after transplantation but statistically not significant. However the corneal thickness at 1 month and after then there were no statistically significant differences. Endothelial cell counts were decreased to 40.3 ± 11.47 %, 42.5 ± 7.39 %, 52.3 ± 11.14 % at 3, 6, and 12 months after transplantation respectively. There were statistically significant differences between preoperative and postoperative cell densities (p < 0.01, anova test). However there was no statistically significant difference between 3 months and 6 months or 6 months and 12 months.

Conclusions: : The results of Descemet’s Membrane Endothelial Keratoplasty in xenografts was worse than allograft. However there was no rejection during 12 months follow up period. Reduced donor tissue volume and anterior chamber associated immune deviation would contribute in the xenotransplantation partly.

Keywords: cornea: endothelium • transplantation 
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