April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Potential Role of Soluble Dj-1 Oncoprotein as Predictive Factor of Growth in Choroidal Nevus
Author Affiliations & Notes
  • M. Bande, Sr.
    Ophthalmology, Hospital Conxo (Provincial), Santiago de Compostela, Spain
  • M. Santiago
    Ophthalmology, Hospital Conxo (Provincial), Santiago de Compostela, Spain
  • P. Mera
    Ophthalmology, Hospital Conxo (Provincial), Santiago de Compostela, Spain
  • M. Blanco
    Ophthalmology, Hospital Conxo (Provincial), Santiago de Compostela, Spain
  • C. Capeans
    Ophthalmology, Hospital Conxo (Provincial), Santiago de Compostela, Spain
  • A. Pineiro
    Ophthalmology, Hospital Conxo (Provincial), Santiago de Compostela, Spain
  • M. Pardo
    Laboratorio de Endocrinología Molecular y Celular, Instituto de Investigación Sanitaria de Santiago de Compostela, Hospital Clínico Universitario de Santiago, Santiago de Compostela, Spain
  • Footnotes
    Commercial Relationships  M. Bande, Sr., None; M. Santiago, None; P. Mera, None; M. Blanco, None; C. Capeans, None; A. Pineiro, None; M. Pardo, None.
  • Footnotes
    Support  Conselleria de Sanidade (Xunta de Galicia) PS09/7
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 867. doi:
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      M. Bande, Sr., M. Santiago, P. Mera, M. Blanco, C. Capeans, A. Pineiro, M. Pardo; Potential Role of Soluble Dj-1 Oncoprotein as Predictive Factor of Growth in Choroidal Nevus. Invest. Ophthalmol. Vis. Sci. 2010;51(13):867.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : In the past decade, many of the choroidal melanoma molecular pathogenesis evidences have emerged to reveal a complex network of molecular events responsible of primary tumor oncogenesis and progression to malignant phenotypes. Nevertheless, choroidal nevi risk of transformation into malignant choroidal melanoma is still unpredictable. We have recently identified the in vitro secretion of DJ-1 oncoprotein from choroidal melanoma tumors and more interestingly, we have proved the possibility to detect this protein in the serum of certain choroidal melanoma patient's serum (Pardo et al., 2006, 2007). The purpose of this study is to correlate known ultrasonographic factors predictive of growth with DJ-1 serum levels in choroidal nevi patient sera.

Methods: : Predictive ultrasonographic factors were analyzed in choroidal nevi patients (n=30) by 20 and 10 MHz ultrasonography following the criteria from the COMS. Additionally, other clinical features associated to poorer risk such as symtoms, orange pigment or drusens were also examined. Serum levels of DJ-1 protein were analyzed in the mentioned patients and in healthy age/sex matched volunteers (n=25) by quantitative sandwich enzyme immunoassay (ELISA).

Results: : While no statistically significant difference was found in DJ-1 serum concentration between choroidal nevi patients and healthy individuals (mean 65.9 ng/ml vs. 41.5 ng/ml; p >.05), DJ-1 variations were found within the choroidal group of patients. Interestingly, DJ-1 serum levels in these patients were significantly higher in those nevi with tumor thickness >1.5 mm (p < .003), basal diameter > 8 mm ( p < .03 ) and ultrasonographic hollowness ( p < .0001 ).

Conclusions: : Elevated DJ-1 oncoprotein serum levels correlate with ultrasound risk factors in choroidal nevi. The protein DJ-1 seems to be a promising factor predictive of growth in choroidal nevi and a potential biomarker for malignancy.

Keywords: choroid • melanoma • tumors 
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