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H.-K. Kuo, P.-C. Wu, Y.-C. Wu; Defocus Shockwave on the Anti-Proliferation in Cultured B16F10 Melanocytes. Invest. Ophthalmol. Vis. Sci. 2010;51(13):873.
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© ARVO (1962-2015); The Authors (2016-present)
This study investigates the anti-proliferative effect of physical shockwave (SW) on the melanocytes.
Mouse B16F10 melanocyte (B16F10) was used for in-vitro study. SW treatment using a defocus shockwave device Orthowave 180 (MTS, Konstanz, Germany) was applied. Effect of SW on the cell proliferation was checked with cell counting. Apoptosis was checked with Western blot for bcl-2 and bax.
Higher shock-wave energy and dose caused more suppression of cell proliferation. 21 kv/600 impulses caused 50% suppression of B16F10 cellular proliferation at 24 hours. The effect of suppression of cell proliferation by high-dose shockwave maintained for 6 days. Shock-wave treatment suppressed the production of bcl-2 and promoted that of bax of B16F10 cells.
Shockwave suppressed B16F10 melanocyte proliferation and induced apoptosis. The degree paralleled to the dose of shockwave.
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