Abstract
Purpose: :
This study investigates the anti-proliferative effect of physical shockwave (SW) on the melanocytes.
Methods: :
Mouse B16F10 melanocyte (B16F10) was used for in-vitro study. SW treatment using a defocus shockwave device Orthowave 180 (MTS, Konstanz, Germany) was applied. Effect of SW on the cell proliferation was checked with cell counting. Apoptosis was checked with Western blot for bcl-2 and bax.
Results: :
Higher shock-wave energy and dose caused more suppression of cell proliferation. 21 kv/600 impulses caused 50% suppression of B16F10 cellular proliferation at 24 hours. The effect of suppression of cell proliferation by high-dose shockwave maintained for 6 days. Shock-wave treatment suppressed the production of bcl-2 and promoted that of bax of B16F10 cells.
Conclusions: :
Shockwave suppressed B16F10 melanocyte proliferation and induced apoptosis. The degree paralleled to the dose of shockwave.
Keywords: melanoma • melanocytes • apoptosis/cell death