April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Defocus Shockwave on the Anti-Proliferation in Cultured B16F10 Melanocytes
Author Affiliations & Notes
  • H.-K. Kuo
    Ophthalmology, Chang Gung Memorial Hospital--Kaohiung Medical Center, Kaohsiung Hsien, Taiwan
    Chang-Gung University,College of Medicine, Kaohsiung, Taiwan
  • P.-C. Wu
    Ophthalmology, Chang Gung Memorial Hospital--Kaohiung Medical Center, Kaohsiung Hsien, Taiwan
    Chang-Gung University, College of Medicine, Kaohsiung, Taiwan
  • Y.-C. Wu
    Ophthalmology, Chang Gung Memorial Hospital--Kaohiung Medical Center, Kaohsiung Hsien, Taiwan
  • Footnotes
    Commercial Relationships  H.-K. Kuo, None; P.-C. Wu, None; Y.-C. Wu, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 873. doi:
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    • Get Citation

      H.-K. Kuo, P.-C. Wu, Y.-C. Wu; Defocus Shockwave on the Anti-Proliferation in Cultured B16F10 Melanocytes. Invest. Ophthalmol. Vis. Sci. 2010;51(13):873.

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Abstract

Purpose: : This study investigates the anti-proliferative effect of physical shockwave (SW) on the melanocytes.

Methods: : Mouse B16F10 melanocyte (B16F10) was used for in-vitro study. SW treatment using a defocus shockwave device Orthowave 180 (MTS, Konstanz, Germany) was applied. Effect of SW on the cell proliferation was checked with cell counting. Apoptosis was checked with Western blot for bcl-2 and bax.

Results: : Higher shock-wave energy and dose caused more suppression of cell proliferation. 21 kv/600 impulses caused 50% suppression of B16F10 cellular proliferation at 24 hours. The effect of suppression of cell proliferation by high-dose shockwave maintained for 6 days. Shock-wave treatment suppressed the production of bcl-2 and promoted that of bax of B16F10 cells.

Conclusions: : Shockwave suppressed B16F10 melanocyte proliferation and induced apoptosis. The degree paralleled to the dose of shockwave.

Keywords: melanoma • melanocytes • apoptosis/cell death 
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