April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Analysis of Lipid Extraction From Hydrogel and Silicone Hydrogel Contact Lenses
Author Affiliations & Notes
  • A. D. Pucker
    College of Optometry, The Ohio State University, Columbus, Ohio
  • M. Thangavelu
    College of Optometry, The Ohio State University, Columbus, Ohio
  • J. J. Nichols
    College of Optometry, The Ohio State University, Columbus, Ohio
  • Footnotes
    Commercial Relationships  A.D. Pucker, None; M. Thangavelu, None; J.J. Nichols, Vistakon, F; CIBA Vision, F; Alcon, F; Inspire, F; Alcon, C; Vistakon, C.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1509. doi:
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    • Get Citation

      A. D. Pucker, M. Thangavelu, J. J. Nichols; Analysis of Lipid Extraction From Hydrogel and Silicone Hydrogel Contact Lenses. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1509.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To understand the propensity of various soft contact lenses for deposition of common tear film lipids.

Methods: : Ten unworn etafilcon A, galyfilcon A, senofilcon A, and lotrafilcon A lenses were individually soaked in 1.0 mL of 1.75 µg/mL cholesterol oleate or 1.0 mL of 0.5 µg/mL phosphatidylcholine solution for 8 hours and 14 days. Each lens was then removed from the incubation solution, and the deposited lipids were extracted with a 1:1 chloroform-methanol extraction. Individual lipid extracts underwent cholesterol oleate and inorganic phosphate assay quantification for each lens extract from each time point (all values for both lipid types in µg/lens).

Results: : For cholesterol oleate, etafilcon A had significantly more at 8 hours than at 14 days but lotrafilcon A had significantly more at 14 days than 8 hours (both p = 0.005). Galyfilcon A and senofilcon A had no difference between the two time points (p = 0.80 and 0.57, respectively). There was no difference in the four materials after 8 hours (range = 3.95 to 4.80). At 14 days, the highest to lowest levels were as follows (p senofilcon A (4.98) = galyfilcon A (4.39) > etafilcon A (2.75). For phosphatidylcholine, senofilcon A had more deposited after 14 days compared to 8 hours (p = 0.02). There were no time point differences for the other materials. At 8 hours, the highest to lowest levels were as follows (p galyfilcon A (1.99) > etafilcon A (1.15) > senofilcon A (0.78). At 14 days, the highest to lowest levels was as follows: lotrafilcon A (4.12) > galyfilcon A (1.47) = etafilcon A (1.83) = senofilcon A (1.14).

Conclusions: : In general, hydrogel and silicone hydrogel contact lenses appear to saturate relatively quickly with these lipids (i.e., within the first 8 hours). While there were material differences in deposition, lotrafilcon A appears to deposit these lipids the most whereas etafilcon A deposits these lipids the least.

Keywords: lipids • cornea: tears/tear film/dry eye • cornea: basic science 
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