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L. C. Huang, A. Agarwal, L. Crawford; In vitro Biocompatibility Assessment of Contact Lens Multi-Purpose Solutions With Silicone Hydrogel Lenses. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1523.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate potential cytotoxic effects of six contact lens multipurpose solutions (MPS) and assess biocompatibility of MPS-cycled silicone hydrogel (SiHy) contact lenses (CLs) on human corneal epithelial cells (HCEC) and mouse fibroblasts.
SV40-transformed HCEC were exposed to six MPS [Complete MPS Easy Rub formula (CER) and an investigational MPS (AMO), Optifree Express, Optifree RepleniSH (Alcon), AQuify (Ciba Vision), Renu Multiplus (Bausch & Lomb)] for 5-30min. Cell viability was determined by MTT assay. Four SiHy CLs [Acuvue Oasys (Vistakon), PureVision (Ciba Vision), Air Optix (Bausch & Lomb), Biofinity (CooperVision)] were cycled in MPS, every 24hrs for 7 days. Monolayers of L929 mouse fibroblasts and HCEC were exposed to cycled CLs for 24hrs. Cells were stained with Trypan Blue and scored for reactivity according to USP Direct Contact Test criteria.
CER, investigational MPS, AQuify, and ReNu MPS exposed HCEC demonstrated similar cell viability as cells exposed to media and PBS controls. Both Optifree MPS significantly decreased HCEC viability (n=3, p<0.05). Direct contact analysis on L929 cells showed adequate compatibility of all CLs with CER, investigational MPS, AQuify, and Renu MPS, with the lowest grade (0) obtained with Biofinity lens cycled in investigational MPS. Both Optifree MPS-cycled CLs demonstrated moderate to severe cytotoxic activity on L929 cells. CLs cycled in CER, AQuify, and investigational MPS did not demonstrate any cytotoxic effects on HCEC. ReNu MPS-cycled Acuvue Oasys and Biofinity CLs showed moderate (grade 3) cytotoxicity whereas ReNu MPS-cycled PureVision and Air Optix CLs exhibited no cytotoxic activity. Both Optifree MPS-cycled CLs displayed moderate to severe cytotoxic effects (grade 3-4) on HCEC.
This study illustrated that CER, investigational MPS, AQuify, and ReNu MultiPlus MPS performed better in maintaining cell viability. Analysis of biocompatibility between SiHy CLs and MPS by direct contact test showed a general consensus between L929 mouse fibroblasts and HCEC. In both cell populations, SiHy CLs were compatible with CER, AQuify, and investigational MPS, moderately compatible with ReNu MultiPlus MPS, and less compatible with Optifree MPS.
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