April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Effects of Multi-Purpose Contact-Lens Care Solutions and Their Ingredients on Membrane-Associated Mucins at the Corneal Epithelial Cells
Author Affiliations & Notes
  • M. Imayasu
    R & D Center, Menicon Co Ltd, Kasugai, Japan
  • Y. Hori
    Ophthalmology, Toho Univ Sakura Med Ctr, Sakura, Japan
  • H. D. Cavanagh
    Ophthalmology, Univ Texas Southwestern Med Ctr, Dallas, Texas
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1525. doi:
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    • Get Citation

      M. Imayasu, Y. Hori, H. D. Cavanagh; Effects of Multi-Purpose Contact-Lens Care Solutions and Their Ingredients on Membrane-Associated Mucins at the Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1525.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Membrane-associated mucins play an important role for protecting from invasion of pathogens at the ocular surface. The purpose of this study was to determine whether multi-purpose contact-lens care solutions (MPSs) and their ingredients regulate the expression of membrane-associated mucins in human corneal epithelial cells.

Methods: : SV40-immortalized human corneal epithelial cells (HCE-T cells) were cultured in DMEM/F12 medium with 5% fetal bovine serum to confluence, and then cultured with 10% five different MPSs, or their representative ingredients, 5ppm polyhexamethylene biguanide (PHMB), 0.1% macrogolglycerol hydroxystearate, 0.1% poloxamer, 0.1% poloxamine, or 0.1% boric acid for 24 hours. Quantitative real-time PCR was used to investigate the gene expression of MUC1, MUC4, and MUC16. Immuno-fluorescence of MUC16 protein on the surface of the HCE-T cells was observed by confocal laser scanning microscope imaging.

Results: : Two MPSs without boric acid had no effects on gene and protein expression of membrane-associated mucins. Three MPSs containing boric acid reduced gene expressions of MUC1, MUC4 and MUC16 to 22-60%, 34-45% and 62-68%, respectively. Immuno-fluorescence microscopy demonstrated that these MPSs reduced the expression of MUC16 protein. Among ingredients, boric acid reduced gene expressions of MUC1, MUC4 and MUC16 to 7%, 56%, 19%, respectively.

Conclusions: : MPSs containing boric acid down-regulate membrane-associated mucins as compared to MPSs that do not contain boric acid. There may be some subtle interactions between ingredients in these solutions that can affect mucins.

Keywords: cornea: epithelium • contact lens • gene/expression 
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