April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Coating of Hydrogel Contact Lenses With Methacrylic Acid Has Differential Effects on P. aeruginosa Attachment
Author Affiliations & Notes
  • V. B. Tran
    Chemical Engineering, Univ of California, Berkeley, Berkeley, California
  • S. M. Fleiszig
    School of Optometry, Univ of California - Berkeley, Berkeley, California
  • D. J. Evans
    School of Optometry, Univ of California - Berkeley, Berkeley, California
    College of Pharmacy, Touro University-California, Vallejo, California
  • C. J. Radke
    Chemical Engineering, Univ of California, Berkeley, Berkeley, California
  • Footnotes
    Commercial Relationships  V.B. Tran, None; S.M. Fleiszig, None; D.J. Evans, None; C.J. Radke, Alcon Labs, F.
  • Footnotes
    Support  NSF Graduate Fellowship (VBT), NEI EY011221(SMJF), Alcon (CJR)
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1532. doi:
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      V. B. Tran, S. M. Fleiszig, D. J. Evans, C. J. Radke; Coating of Hydrogel Contact Lenses With Methacrylic Acid Has Differential Effects on P. aeruginosa Attachment. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1532.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : P. aeruginosa

Methods: : Membranes made of contact-lens material were synthesized by UV-initiated polymerization of HEMA with cross-linker (EGDMA), water, with or without MAA. Synthesized membranes had a high MAA content (70:30 MAA to HEMA volume ratio). Mian’s minimal media containing 5 x106 cfu/ml P. aeruginosa strains PAO1 or PAK was pumped through a flow chamber containing a mounted membrane for 1 h at a constant shear rate (0.3 s-1). Attachment dynamics were visualized and quantified by phase-contrast video microscopy

Results: : PAK wild-type bacteria bound to HEMA membranes at 230 bacteria/mm2-min. However membranes with MAA bound only 5 bacteria/mm2-min. (98 % reduction, p = 0.01, t-Test). P. aeruginosa, PAO1, adhered at a rate of 500 bacteria/mm2/min to HEMA membranes, and at a similar rate of 550 bacteria/mm2-min to HEMA-MAA membranes. (p = 0.15, t-Test).

Conclusions: : MAA coating had vastly different, strain-dependent, effects on P. aeruginosa attachment to hydrogel contact lens material in this model. These differences likely reflect variations in attachment strategies used by bacteria (even within the same species), and highlight the complexity involved in designing a universal strategy for reducing bacterial attachment to contact lenses.

Keywords: pseudomonas • contact lens • microscopy: light/fluorescence/immunohistochemistry 
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