April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Role of B7-H3/TLT-2 Pathway on Corneal Allograft Survival
Author Affiliations & Notes
  • H. Taniguchi
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • Y. Kitahara
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • M. Oshima
    Immunology, National Institute of Infectious Disease, Tokyo, Japan
  • H. Akiba
    Immunology, Juntendo University School of Medicine, Tokyo, Japan
  • H. Yagita
    Immunology, Juntendo University School of Medicine, Tokyo, Japan
  • M. Azuma
    Molecular Immunology, Tokyo Medical and Dental University, Tokyo, Japan
  • J. Hori
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • Footnotes
    Commercial Relationships  H. Taniguchi, None; Y. Kitahara, None; M. Oshima, None; H. Akiba, None; H. Yagita, None; M. Azuma, None; J. Hori, None.
  • Footnotes
    Support  Grant-in-Aid for Scientific Research(C) from the Japan Society for the Promotion of Science
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1550. doi:
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      H. Taniguchi, Y. Kitahara, M. Oshima, H. Akiba, H. Yagita, M. Azuma, J. Hori; Role of B7-H3/TLT-2 Pathway on Corneal Allograft Survival. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1550.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We have reported that B7-H3, a costimulatory signal B7 family molecule, is an essential molecule for acceptance of corneal allografts and induction of anterior chamber-associated immune deviation (ACAID). Recently, Triggering receptor expressed on myeloid cells-like transcript-2 (TLT-2) has been identified as a B7-H3 receptor. This study analyzed the intraocular expression of B7-H3. In addition, we analyzed the inhibition and molecular function of the B7-H3/TLT-2 pathway using 3 different antibodies in a corneal transplant model.

Methods: : Expression of B7-H3 was assessed by RT-PCR in the cornea, iris-ciliary body, and retina of normal mouse eyes. Normal corneas of C57BL/6 were transplanted orthotopically into normal eyes of BALB/c mice. Anti-B7-H3 monoclonal antibody (mAb) (MJ18) (which does not inhibit binding to TLT-2), anti-B7-H3 mAb (M1H35) (which does inhibit binding to TLT-2), anti-TLT-2 mAb (M1H49), or control rat IgG were administered intraperitoneally, and allograft acceptance was analyzed.

Results: : B7-H3 was constitutively expressed in the cornea, iris-ciliary body, and retina of normal eyes. In groups treated with anti-B7-H3 mAbs or anti-TLT-2 mAb, all allografts were rejected. Compared to the control group, survival was significantly shortened (p<0.05). Compared to anti-B7-H3 mAb (MJ18) that did not inhibit binding to TLT-2, anti-B7-H3 mAb (M1H35) that did inhibit binding to TLT-2 significantly shortened survival.

Conclusions: : B7-H3 is constitutively expressed in ocular tissue. B7-H3/TLT-2 pathway plays a suppressive role in immune response of the corneal allografts. B7-H3 expressed in the ocular tissue may facilitate allograft survival.

Keywords: immune tolerance/privilege • transplantation • immunomodulation/immunoregulation 
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