Abstract
Purpose: :
To observe the effect of Avastin eyedrops on immune rejection after high-risk keratoplasty in rabbits.
Methods: :
Alkali-burn was used to establish neovascularization animal model. Allograft corneal transplantation was performed. . Avastin group: 0.25% Avastin eyedrops were used once a day for four weeks; at the same time 0.3% ofloxcine eyedrops was applied on control group. The time and degree of rejection were observed, and CD 4+ ,CD8+ cells infiltration were also detected in the rejected grafts.
Results: :
Corneal grafts of 5 eyes in control group were rejected within 24 days, the mean time of rejection is 20.6 days; 4 grafts were rejected in Avastin group more than one month after operation, the mean time is 33 day. the difference between two groups is statistical significance.
Conclusions: :
Avastin local application can delay immune rejection after penetrating keratoplasty in rabbits.
Keywords: cornea: stroma and keratocytes • cornea: epithelium • cornea: surface mucins