April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Innate T Cells Are Necessary for Maximal Expression of Allergic Conjunctivitis
Author Affiliations & Notes
  • N. J. Reyes
    Ophthalmology, UT Southwestern Medical Center at Dallas, Dallas, Texas
  • E. Mayhew
    Ophthalmology, UT Southwestern Medical Center at Dallas, Dallas, Texas
  • P. W. Chen
    Ophthalmology, UT Southwestern Medical Center at Dallas, Dallas, Texas
  • J. Y. Niederkorn
    Ophthalmology, UT Southwestern Medical Center at Dallas, Dallas, Texas
  • Footnotes
    Commercial Relationships  N.J. Reyes, None; E. Mayhew, None; P.W. Chen, None; J.Y. Niederkorn, None.
  • Footnotes
    Support  NIH Grant EY0007641 and EY016664 and an unrestricted grant from Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1567. doi:
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    • Get Citation

      N. J. Reyes, E. Mayhew, P. W. Chen, J. Y. Niederkorn; Innate T Cells Are Necessary for Maximal Expression of Allergic Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1567.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : While Th2 cells have been studied in allergic conjunctivitis, the role of innate T cells has not been determined. We determined the function of NKT and γΔ T cells in early and late phase responses in allergic conjunctivitis.

Methods: : Wild-type (WT) C57BL/6, NKT deficient (CD1d-/-), and γΔ T cell deficient (TCR-Δ-/-) mice were immunized intraperitoneally and challenged topically for seven consecutive days with short ragweed (SRW) pollen. Control mice were challenged topically with PBS. NKT and γΔ T cell double deficient mice were generated by treating TCR-Δ-/- mice with anti-CD1d antibody. Early phase responses of allergic conjunctivitis were evaluated clinically and late phase responses by histopathology. Cytokine profiles were evaluated by ELISA.

Results: : SRW-sensitized CD1d-/- mice and TCR-Δ-/- mice had decreased tearing, lid edema, conjunctival edema and vasodilatation compared to SRW-treated WT mice. Both SRW-treated CD1d-/- mice and TCR-Δ-/- mice had decreased conjunctival eosinophil infiltration compared to SRW-treated WT mice. SRW-treated NKT cell- and γΔ T cell-deficient mice displayed a reduction in the production of Th2-associated cytokines IL-4, IL-5 and IL-13 compared to WT mice (P<0.05). Early phase of allergic conjunctivitis was lowest in SRW-treated NKT cell-depleted, TCR-Δ-/- mice compared to control TCR-Δ-/- mice (P<0.05). However, late phase of allergic conjunctivitis in SRW-treated NKT cell-depleted, TCR-Δ-/- mice was the same as SRW pollen-treated TCR-Δ-/- mice; both groups had comparable levels of conjunctival eosinophil infiltration (P>0.05).

Conclusions: : NKT and γΔ T cells are needed for full expression of allergic conjunctivitis. Elimination of either T cell population decreases the severity of both early and late phase responses. Elimination of both NKT and γΔ T cell populations has an additive effect in reducing early phase responses, but has no effect on late phase responses suggesting that these cells affect allergen-specific IgE production. Reduction in early and late phase responses correlates with decreased production of Th2 cytokines. Thus, innate T cells have an important impact in the expression of allergic conjunctivitis.

Keywords: immunomodulation/immunoregulation • inflammation • immune tolerance/privilege 
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