April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Nasolacrimal Duct Closure Modulates Ocular Mucosal and Systemic CD4+ T Cell Responses Induced Following Topical Ocular or Intranasal Immunization
A. B. Nesburn; A. A. Chentoufi; G. Dasgupta; I. Bettahi; N. R. Binder; Z. S. Choudhury; W. D. Chamberlain; S. L. Wechsler; L. BenMohamed
Author Affiliations & Notes
  • A. B. Nesburn
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • A. A. Chentoufi
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • G. Dasgupta
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • I. Bettahi
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • N. R. Binder
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • Z. S. Choudhury
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • W. D. Chamberlain
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • S. L. Wechsler
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • L. BenMohamed
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • Footnotes
    Commercial Relationships  A.B. Nesburn, None; A.A. Chentoufi, None; G. Dasgupta, None; I. Bettahi, None; N.R. Binder, None; Z.S. Choudhury, None; W.D. Chamberlain, None; S.L. Wechsler, None; L. BenMohamed, None.
  • Footnotes
    Support  Supported by Public Health Service research grants EY14900, EY14017, EY15225, and EY16663 from the NIH, by the Discovery Eye Foundation, and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1572. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Nasolacrimal Duct Closure Modulates Ocular Mucosal and Systemic CD4+ T Cell Responses Induced Following Topical Ocular or Intranasal Immunization
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      A. B. Nesburn, A. A. Chentoufi, G. Dasgupta, I. Bettahi, N. R. Binder, Z. S. Choudhury, W. D. Chamberlain, S. L. Wechsler, L. BenMohamed; Nasolacrimal Duct Closure Modulates Ocular Mucosal and Systemic CD4+ T Cell Responses Induced Following Topical Ocular or Intranasal Immunization. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1572.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : Both topical ocular and topical intranasal immunizations have been reported to stimulate the ocular mucosal immune system (OMIS) and the systemic immune system. Nasolacrimal ducts (NLDs) are the connecting bridges between OMIS and nasal associated lymphoid tissue (NALT). These ducts drain topical ocular administrated solutions into the inferior meatus of the nose to reach the NALT. Inversely, NLDs also drain intranasally administrated solutions to the mucosal surface of eye and thus the OMIS. This unique anatomical connection between the OMIS and NALT systems provoked us to test whether OMIS and NALT are immunologically interdependent.

Methods: : CD4+ T cell epitope peptides from HSV-1 glycoprotein D (gD) emulsified with the mucosal adjuvant CpG2007 were used to immunize rabbits either ocularly or intranasally. In some rabbits the ocular and intranasal compartments were isolated by surgical closure of NLDs.

Results: : Topical ocular and topical intranasal administration of the vaccine induced local (conjunctival) as well as systemic (spleenic) HSV-peptide-specific CD4+ T cell responses. Topical ocular immunization in rabbits with sealed NLDs induced local ocular mucosal CD4+ T cell responses similar to those induced in normal rabbits, while systemic CD4+ T cell responses were significantly increased (as measured by T cell proliferation and IFN-γ production; p < 0.005). In contrast, topical intranasal immunization in rabbits with sealed NLDs induced systemic CD4+ T cell responses similar to those induced in normal rabbits, while ocular mucosal immune responses were significantly decreased (p < 0.001).

Conclusions: : These studies confirm that both topical ocular and topical intranasal administration of HSV-1 epitopes can induce local and systemic immune responses. In addition, the results suggest that NALT and OMIS are immunologically interconnected.

Keywords: immunomodulation/immunoregulation • conjunctiva • herpes simplex virus 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept