April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
The Gp130/STAT3 Pathway Regulates Adaptive Photostasis
Author Affiliations & Notes
  • J. Wang
    Ophthalmology, Univ Oklahoma Hlth Sci Ctr, Oklahoma City, Oklahoma
  • S. Chollangi
    Department of Bioengineering, The University of Oklahoma, Norman, Oklahoma
  • A. Saadi
    Cell Biology,
    University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
  • J. Ash
    University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
  • Footnotes
    Commercial Relationships  J. Wang, None; S. Chollangi, None; A. Saadi, None; J. Ash, None.
  • Footnotes
    Support  R01 EY016459,EY02422, P20 RR017703, P30 EY012190, Foundation Fighting Blindness and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1680. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J. Wang, S. Chollangi, A. Saadi, J. Ash; The Gp130/STAT3 Pathway Regulates Adaptive Photostasis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1680. doi: https://doi.org/.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Photostasis is an adaptive response of photoreceptors to the stress of prolonged exposure to bright cyclic light. Animals reared in relatively bright cyclic light have shortened and disorganized outer segments, with decreased rhodopsin packing density in the disk membrane which will reduce efficiency in photon capture by rhodopsin. Our purpose was to identify the molecular mechanisms regulating photostasis in mouse retina.

Methods: : To test the role of gp130 and STAT3 in photostasis we used mice with retina-specific knockout of either gp130 or STAT3. Two methods, preconditioning mice for 6 days in cyclic bright-light or Intravitreal injections of leukemia inhibitory factor (LIF) were used to induce photostasis. Photostasis was measured by ERG, histology, and expression of phototransduction proteins and mRNA. Chromatin immunoprecipitation was used to analyze STAT3 binding to the promoters of phototransduction related genes.

Results: : Both preconditioning with bright light and intravitreal injections of LIF reduced a-waves by ERG, reduced outer segment length, and reduced phototransduction protein and gene expression. In both models STAT3 was strongly activated during photostasis. Deletion of gp130 or STAT3 in the retina blocked the induction of photostasis in both models. Chromatin immunoprecipitation demonstrated STAT3 binding to promoter elements in phototransduction related genes.

Conclusions: : Results from this study demonstrated that both gp130 and STAT3 are necessary for adaptive photostasis. STAT3 binding to DNA near phototransduction genes suggests that STAT3 is functioning to suppress their expression resulting in photostasis.

Keywords: neuroprotection • photoreceptors • injection 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.