April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Detecting Acute Inner Retinal Excitotoxic Injury in Mice Using Diffusion MRI
Author Affiliations & Notes
  • J. Chen
    Internal Medicine,
    Washington University, St. Louis, Missouri
  • C.-W. Chiang
    Washington University, St. Louis, Missouri
  • S.-K. Song
    Washington University, St. Louis, Missouri
  • Footnotes
    Commercial Relationships  J. Chen, None; C.-W. Chiang, None; S.-K. Song, None.
  • Footnotes
    Support  NIH R21 EY018914; Washington University DRTC DK20579
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1885. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J. Chen, C.-W. Chiang, S.-K. Song; Detecting Acute Inner Retinal Excitotoxic Injury in Mice Using Diffusion MRI. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1885.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Cerebral excitotoxic injury leads to acute decrease of apparentdiffusion coefficient (ADC) detected by in vivo diffusion MRI.We examined the feasibility of detecting retinal excitotoxicinjury using the same technique.


Forty-five 2-month old male C57BL/6 mice were imaged in vivoat 11.7T followed by cross-sectional histology using previouslyreported method1. Each mouse was examined at baseline, 3-hour,1-, 3-, or 7-day post intravitreal injection of 0.5uL 5mM NMDAor 0.5uL saline (n>=5 for each group except n=3 for saline3- and 7-day). The ADC map was calculated from diffusion weightedimages. The three MR-detected retinal layers were respectivelyassigned to NFL/GCL/IPL, INL/OPL, and ONL/IS/OS.


NMDA excitotoxicity resulted in substantial retinal swellingat 1-day (Fig. 1). However, an acute increase of inner retinaldiffusion weighted MR signal (Fig 1) reflecting a 30% decreaseof ADC in INL/OPL (Fig. 2) was observed as early as 3-hour,consistent with previous report in cerebral cytotoxic edema.ADC in NFL/GCL/IPL exhibited a transient increase suggestingdeath of ganglion cells that are sensitive to NMDA excitotoxicity.ADC in ONL/IS/OS was unchanged at all times because the pre-synapticphotoreceptor cells are resistant to NMDA excitotoxicity.


Decreased ADC was observed before detectable retinal swellingin NMDA induced retinal excitotoxic injury.1.Chen J et. al,Magn Reson Med, 2008  


Keywords: retina • edema • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.