April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Vitreo-Retinal Interface (VRI) Injury After Acceleration Impulse in a Porcine Model
R. Mandiga; T. L. Gosen; S. S. Huang
Author Affiliations & Notes
  • R. Mandiga
    Department of Ophthalmology and Visual Sciences, University Hospitals Eye Institute, Cleveland, Ohio
    Department of Ophthalmology, Case Western Reserve University School of Medicine, Cleveland, Ohio
  • T. L. Gosen
    Department of Ophthalmology, Case Western Reserve University School of Medicine, Cleveland, Ohio
  • S. S. Huang
    Department of Ophthalmology and Visual Sciences, University Hospitals Eye Institute, Cleveland, Ohio
    Department of Ophthalmology, Case Western Reserve University School of Medicine, Cleveland, Ohio
  • Footnotes
    Commercial Relationships  R. Mandiga, None; T.L. Gosen, None; S.S. Huang, SurModics, Inc., I; Digital Healthcare, Inc., I; University Hospitals Eye Institute, E; Philip F. and Elizabeth G. Searle – Suber Huang Chair Professorship, E; i2i Innovative Ideas, Inc., E; Bausch & Lomb, C; Merck & Co., Inc., C; SurModics, Inc., C; Second Sight, C; Therapeutic Nanoparticle and Molecular Imaging, C; Digital Healthcare, Inc., C; American Academy of Family Physicians, C; Retinal Dis Image Analysis Reading Center/Case, C; Neurotech – REDIARC Fundus Reading, C; Lux Bio – REDIARC Fundus Reading, C; Alcon – REDIARC Fundus Reading, C; Pfizer – REDIARC Fundus Reading, C; Schering Plough – REDIARC Fundus Reading, C; VRT – Vitreo Retinal Technologies – REDIARC, C; American Retina Foundation, C; Diabetic Retinopathy Clinical Research Network (DRCR), C; MacuSight – REDIARC Fundus Reading, C; American Academy of Ophthalmology, C; NEHEP/NEI/NIH, C.
  • Footnotes
    Support  SEARLE Retinal Research Endowment
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1889. doi:
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      R. Mandiga, T. L. Gosen, S. S. Huang; Vitreo-Retinal Interface (VRI) Injury After Acceleration Impulse in a Porcine Model. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1889.

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Abstract

Purpose: : To identify a threshold for VRI injury after anterior-posterior (A-P) and lateral vector acceleration impulse in a fresh porcine globe model.

Methods: : Fresh porcine globes were shipped overnight from Sioux-Preme Packaging (Sioux City, IA) on ice within 24 hours of harvest. As per our protocol to generate a model that mimicked human adult vitreous, each eye was injected with 0.3cc of 0.25% trypsin-EDTA (Invitrogen Carlsbad, CA) and incubated for 2 hours. A pendulum induced A-P or lateral vector acceleration impulse on each globe suspended within a housing box attached to an Endevco 751-10 accelerometer (San Juan Capistrano, CA) to generate impulses of 60g, 100g, 200g, 300g, 400g, and 500g. Each globe was dissected and inspected for gross VRI injury.

Results: : In the A-P vector group, 10% of the eyes at 60g (n=10), 20% at 100g (n=10), 60% at 200g (n=10), 54.5% at 300g (n=11), 80% at 400g (n=15) and 90% at 500g eyes (n=20) showed VRI injury. Under 400g, VRI injury was limited to 1 quadrant of up to 3 holes at the vitreous base. Above 400g, VRI injury included up to 5 holes, small horseshoe tears, or dialyses in one quadrant of the vitreous base. In the lateral vector group, 10% of the eyes at 60g (n=10), 40% at 100g (n=10), 60% at 200g (n=10), 70% at 300g (n=10), 90% at 400g (n=10) and 90% at 500g (n=10) showed VRI injury. Under 400g, VRI injury included up to 5 holes or multiple tears in multiple quadrants of the vitreous base. Above 400g, VRI injury was extensive from 4 to 16 holes, multiple dialyses, and horseshoe tears located around the vitreous base.

Conclusions: : The threshold for VRI injury lies between 60g and 100g after both A-P and lateral vector acceleration impulse injury and was localized to the vitreous base in our model. The injury pattern varied by impulse vector. A-P impulse consistently produced single quadrant relatively minor VRI injury, while lateral impulse produced more extensive multiple quadrant injury regardless of amplitude. A proposed explanation for more extensive injury in the lateral group is that the eye is better equipped to handle the shearing A-P vector impulse by benefiting from a firm attachment of the vitreous base to the optic nerve head and retinal vessels and a buffering effect of the lens-iris diaphragm.

Keywords: trauma • retinal detachment • vitreous 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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