April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Effects of Oxidative Stress on Conjunctiva/Ocular Surface and Tear Functions in Sod1 (-/-) Mouse
Author Affiliations & Notes
  • T. Kojima
    J&J Ocular Surface and Visual Optics, Keio University School of Medicine, Tokyo, Japan
  • O. M. A. Ibrahim
    J&J Ocular Surface and Visual Optics, Keio University School of Medicine, Tokyo, Japan
  • T. H. Wakamatsu
    J&J Ocular Surface and Visual Optics, Keio University School of Medicine, Tokyo, Japan
  • T. Shimizu
    Research Team for Molecular Biomarkers, Tokyo Metropolitan Insititute of Gerontology, Tokyo, Japan
  • M. Dogru
    J&J Ocular Surface and Visual Optics, Keio Univ School of Medicine, Tokyo, Japan
  • K. Tsubota
    Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships  T. Kojima, None; O.M.A. Ibrahim, None; T.H. Wakamatsu, None; T. Shimizu, None; M. Dogru, None; K. Tsubota, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1918. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T. Kojima, O. M. A. Ibrahim, T. H. Wakamatsu, T. Shimizu, M. Dogru, K. Tsubota; Effects of Oxidative Stress on Conjunctiva/Ocular Surface and Tear Functions in Sod1 (-/-) Mouse. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1918.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Healthy conjunctiva secreting mucins is essential for maintaining the integrity of the ocular surface epithelium. We used the Sod1(-/-) mice deficient in Cu, Zn - superoxide dismutase 1 (SOD1) and investigated the effect of oxidative stress on the conjunctival phenotype/ocular surface health and tear functions.

Methods: : Fifty weeks old C57/B6 wild type mice (wt) and Sod1(-/-) mice were used for evaluations of the tear film break-up time (BUT), fluorescein staining of the cornea, and the periodic acid Schiff (PAS) staining of the conjunctival specimens to detect goblet cell densities in the conjunctiva. Immunohistochemistry staining with anti-MUC5AC and anti-4-hydroxy-2-nonenal (4HNE) antibodies were also performed.

Results: : The mean BUT value was 2.8±1.5s and 3.8±0.8s in the Sod1(-/-) and wt mice, respectively (p< 0.05). The respective corneal fluorescein staining score was 5.0±1.6 and 3.3±1.0 points, respectively (p< 0.05). The conjunctiva of Sod1(-/-) mice showed marked thickening of conjunctival epithelium and keratinization. The goblet cell density in the wt mice was 58.9±24.7 compared to 4.8±5.1 cells/ fornix in the Sod1(-/-) mice, respectively (p< 0.05). The number of cells positively stained with MUC5AC were significantly lower in the Sod1(-/-) mice (2.6±2.7 cells/fornix) compared to the wt mice (12.3±4.9 cells/fornix) (p < 0.05). Conjunctival epithelium in Sod1(-/-) mice displayed marked staining with 4HNE antibodies.

Conclusions: : Marked tear instability with decreased MUC5AC secreting goblet cells, corneal epithelial damage and increased lipid oxidation were the prominent features of the ocular surface disease in the Sod1(-/-) mice. Elevated conjunctival oxidative stress status appears to affect the conjunctival mucin secretions and the epithelial phenotype. Sod1(-/-) mice may serve as a good model for mucin deficient dry eye disease.

Keywords: cornea: tears/tear film/dry eye • oxidation/oxidative or free radical damage • conjunctiva 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×