April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Corneal Epithelial Response to Patterned Silk Film Substrates
Author Affiliations & Notes
  • B. D. Lawrence
    Biomedical Engineering, Cornell University, Ithaca, New York
    Margaret M. Dyson Vision Research Institute, Weill Cornell Medical College, New York, New York
  • Z. Pan
    Margaret M. Dyson Vision Research Institute, Weill Cornell Medical College, New York, New York
  • M. I. Rosenblatt
    Margaret M. Dyson Vision Research Institute, Weill Cornell Medical College, New York, New York
  • Footnotes
    Commercial Relationships  B.D. Lawrence, Bombyx Technologies, Inc., E; Bombyx Technologies, Inc., C; Bombyx Technologies, Inc., P; Z. Pan, None; M.I. Rosenblatt, None.
  • Footnotes
    Support  NIH K08EY015829, R21EY019561, T32EY007138 and R24EY015656. Research to Prevent Blindness Career Development Award. Tri-Institutional Stem Cell Initiative.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1936. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      B. D. Lawrence, Z. Pan, M. I. Rosenblatt; Corneal Epithelial Response to Patterned Silk Film Substrates. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1936.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Silk fibroin is a choice biomaterial for the design of cellular scaffolds for applications related to regenerative medicine. Recently, the use of fibroin films for ophthalmic applications has been under investigation due to their transparent nature and controllable material properties. Of particular interest is the recent finding that silk film surfaces can be patterned to produce a variety of surface topographies. The following study investigates the effects of various silk film geometrical topographies upon corneal epithelium cell adhesion, proliferation, and cell sheet migration.

Methods: : Microfabrication techniques were utilized to manufacture various silicon surfaces. Each patterned surface consisted of 2 um width, 2 um pitch, and 1.5 um depth features. Features were designed to produce various geometrical shapes consisting of lines, concentric rings, and spiral patterns. PDMS negative molds were produced from the silicon pattern, which were then used to cast silk fibroin solution upon. A well characterized cell line, human corneal-limbal epithelium (HCLE), was used to assess initial cell adhesion, culture proliferation, and cell sheet migration effects upon the various topographies, which included both flat silk film surfaces and tissue culture plastic (TCP).

Results: : Cell adhesion studies demonstrated that on average all silk film surfaces exhibited equal cell attachment after a 2 hr culture period; in addition, it was found that TCP had 60% greater cell adherence than silk film substrates. MTT cell proliferation assays indicated that cell number was at 30% TCP levels for silk films after day 1 in culture and increased to equal levels by day 5. Scratch wound cell migration assays indicated that cell sheet movement into the damaged region followed the geometrical pattern orientation, while overall migration rates varied for each substrate.

Conclusions: : Silk films provide a less adhesive surface for initial cell attachment when compared to TCP, while allowing for culture expansion over time that is comparable to TCP. En masse migration is affected by the presented topography. These results indicate that patterned silk films may offer a new biomaterial option in the clinical setting for potential uses in ocular surface repair for expediting epithelial healing after injury.

Keywords: cornea: epithelium • proliferation • regeneration 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.