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M. C. Morales, V. Freire, J. Etxebarria, J. A. Durán, N. Andollo; Human Corneal Cell Proliferation Induced by Different Blood-Derived Preparations. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1940.
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To compare the proliferation pattern of human corneal epithelial (HCE) cells cultured with three different blood-derived preparations.
Whole blood was collected by venipuncture from 16 healthy volunteers (age 30-60). Blood was processed by 3 different methods: 1) centrifugation at 460 g for 8 min (PRP) 2) centrifugation at 460 g for 8 min followed by coagulation (PRGF) and 3) coagulation and centrifugation at 1000 g for 15 min (AS). Cell viability was measured by colorimetric MTT assay.
The results show that two of the preparations (AS and PRGF) produced a dose-dependent growth pattern in HCE cells. In addition, PRGF induced a higher proliferation rate than the other preparations.When comparing cell viability after incubation with each of the three different preparations with the control culture grown with fetal calf serum (FCS), we observed that cultures grown with PRGF showed the highest cell viability in the majority of the times and concentrations studied. Furthermore, only cell viability after culturing with PRGF 50% showed not statistically significant differences in comparison with the control cultures.
A new blood-derived preparation (PRGF) can improve the corneal cell proliferation capacity in culture with a good tolerability.
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