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T. P. Parikh, N. Eisner, S. Seidman, P. Garcia, S. K. Bhattacharya; Proteomic Analysis of Corneal Tissue Following Traumatic Chemical Injury. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1965.
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To simulate chemical eye injuries and determine whether exposure to variable concentrations of alkali for pre-determined time lengths results in predictable changes to the corneal protein profile of bovine and porcine eyes.
Enucleated bovine and porcine eyes were first subjected to Fluorescein staining to confirm corneal epithelial integrity. Bovine (n=50) and porcine (n=50) cornea were then exposed to strong (sodium hydroxide) and weak (ammonium and calcium hydroxide) alkaline chemicals, excised, and subjected to protein extraction. Protein amount was determined by spectrophotometry and profiled by SDS-PAGE analysis.
Alkali exposed corneal tissue resulted in lower protein extractability at all times compared to control tissue. The sequential lower protein yields seen in the initial time points in the exposed eyes are consistent with the observation of protein aggregate formation. Aggregate formation occurred at earlier time points with strong alkali exposure than compared aggregate formation seen with weaker alkali. Later time points show a gradual increase yield of protein consistent with degradation of proteins due to an increased number of protein entities extracted and contributory to absorbance.
Exposure to alkaline chemicals results predictable changes in corneal protein profile. The different duration and strength of exposure shows differences in protein profile. Stronger alkali and duration of exposure results in reproducible changes suggesting severity of exposure can be predicted from corneal protein profile changes.
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