April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
TGF-β2 Signalling Pathway Activation in Keratoconus Corneas
C. Engler; S. Chakravarti; C. G. Eberhart; A. S. Jun
Author Affiliations & Notes
  • C. Engler
    Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland
  • S. Chakravarti
    Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland
  • C. G. Eberhart
    Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland
  • A. S. Jun
    Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  C. Engler, None; S. Chakravarti, None; C.G. Eberhart, None; A.S. Jun, None.
  • Footnotes
    Support  NIH, Medical Illness Counseling Center, Research to Prevent Blindness, Wilmer Scholars Research Fund, Eye Bank Association of America
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1966. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      TGF-β2 Signalling Pathway Activation in Keratoconus Corneas
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      C. Engler, S. Chakravarti, C. G. Eberhart, A. S. Jun; TGF-β2 Signalling Pathway Activation in Keratoconus Corneas. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1966.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : To assess the pathogenetic role of transforming growth factor beta (TGF-β) pathway markers in the epithelium of keratoconus corneas.

Methods: : Immunohistochemistry (IHC) for TGF-β2, TGF-β1/3 and pSMAD2 was performed on formalin fixed, paraffin embedded sections of keratoconus patient corneas and normal, age-matched corneas from human autopsy eyes. Keratoconus corneas were divided in two groups, depending on their severity based on K-Readings and pachymetry. IHC signal quantitation was performed using automated software.

Results: : IHC quantitation showed a significant increase in mean signal in the group of severe keratoconus cases compared to normal corneas for TGF-β2 and pSMAD2. TGF-β1/3 did not show any significant increase in the keratoconus corneas versus the autopsy controls.

Conclusions: : This work shows the involvement of the TGF-β2 pathway in severe keratoconus cases. To elucidate its role in the pathogenesis of keratoconus further studies will be required.

Keywords: keratoconus • immunohistochemistry • pathobiology 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept