April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
TGF-β2 Signalling Pathway Activation in Keratoconus Corneas
Author Affiliations & Notes
  • C. Engler
    Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland
  • S. Chakravarti
    Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland
  • C. G. Eberhart
    Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland
  • A. S. Jun
    Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  C. Engler, None; S. Chakravarti, None; C.G. Eberhart, None; A.S. Jun, None.
  • Footnotes
    Support  NIH, Medical Illness Counseling Center, Research to Prevent Blindness, Wilmer Scholars Research Fund, Eye Bank Association of America
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1966. doi:
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      C. Engler, S. Chakravarti, C. G. Eberhart, A. S. Jun; TGF-β2 Signalling Pathway Activation in Keratoconus Corneas. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1966.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To assess the pathogenetic role of transforming growth factor beta (TGF-β) pathway markers in the epithelium of keratoconus corneas.

Methods: : Immunohistochemistry (IHC) for TGF-β2, TGF-β1/3 and pSMAD2 was performed on formalin fixed, paraffin embedded sections of keratoconus patient corneas and normal, age-matched corneas from human autopsy eyes. Keratoconus corneas were divided in two groups, depending on their severity based on K-Readings and pachymetry. IHC signal quantitation was performed using automated software.

Results: : IHC quantitation showed a significant increase in mean signal in the group of severe keratoconus cases compared to normal corneas for TGF-β2 and pSMAD2. TGF-β1/3 did not show any significant increase in the keratoconus corneas versus the autopsy controls.

Conclusions: : This work shows the involvement of the TGF-β2 pathway in severe keratoconus cases. To elucidate its role in the pathogenesis of keratoconus further studies will be required.

Keywords: keratoconus • immunohistochemistry • pathobiology 
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