April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Erythropoietin Administration Attenuates Pro-Inflammatory Cytokine Production by Müller Cells in Models of Diabetic Retinopathy
Author Affiliations & Notes
  • X. Lei
    Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China
  • J. Zhang
    Tongji Eye Institute and Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, China
  • J. Shen
    Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China
  • L. Hu
    Department of Ophthalmology, Peking Union Medical College, Beijing, China
  • Y. Wu
    Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China
  • L. Mou
    Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China
  • G. Xu
    Department of Ophthalmology, Second Affiliated Hospital of Soochow University, Suzhou, China
  • W. Li
    Department of Ophthalmology, Peking Union Medical College, Beijing, China
    Department of Ophthalmology, Drexel University College of Medicine, Philadelphia, Pennsylvania
  • G.-T. Xu
    Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Tongji Eye Institute and Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, China
  • Footnotes
    Commercial Relationships  X. Lei, None; J. Zhang, None; J. Shen, None; L. Hu, None; Y. Wu, None; L. Mou, None; G. Xu, None; W. Li, None; G.-T. Xu, None.
  • Footnotes
    Support  Sciences and Technology Commission of Shanghai Municipality: 08ZR1422100 and 08410701200; Unrestricted Research Fund from the Clear Vision Foundation, Philadelphia USA.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1988. doi:
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      X. Lei, J. Zhang, J. Shen, L. Hu, Y. Wu, L. Mou, G. Xu, W. Li, G.-T. Xu; Erythropoietin Administration Attenuates Pro-Inflammatory Cytokine Production by Müller Cells in Models of Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1988.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the regulatory effects of erythropoietin (EPO) on the production of pro-inflammatory cytokines by Müller cells under the condition of diabetic retinopathy (DR).

Methods: : In vitro,

Results: : In rMC-1, the expression of TNF-α, IL-1β, IL-6 and VEGF were enhanced by 1mM glyoxal. The elevated TNF-α and IL-1β, but not IL-6 and VEGF, were normalized by 2U/ml EPO. Moreover, the transcriptional activity of AP-1, but not NF-ΚB, was influenced by glyoxal and EPO. In early diabetic rats, these pro-inflammatory cytokines except for VEGF were up-regulated. Treatment with EPO (8mU/ per eye) significantly reduced the proteins of TNF-α and IL-1β and moderately attenuated IL-6 production.

Conclusions: : These results indicate that in both models of DR EPO attenuates inflammatory response via Müller cells by direct inhibition upon production of TNF-α and IL-1β, which may be due to the suppression of AP-1 activation. In contrast, the expression of IL-6 and VEGF in Müller cells is not regulated by EPO. These findings implicate therapeutic potential of EPO in amelioration of retinal inflammation in DR.

Keywords: cytokines/chemokines • Muller cells • diabetic retinopathy 
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