Purchase this article with an account.
K. K. Najafi, S. S. Wilmarth, R. Najafi, B. Khosrovi, M. Anderson, C. Celeri, D. Debabov, M. Chowhan, D. W. Stroman; In vitro Microbiocidal Activity in Tears of N,N-dichloro-2,2-dimethyltaurine (NVC-422, AL-46383A), a Novel Topical Ophthalmic Agent. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1992.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To evaluate the microbicidal activity and chemical stabilityin synthetic tears of N,N-dichloro-2,2-dimethyltaurine (NVC-422),which is a synthetic analog of N-chlorotaurine (NCT). NCT isa relatively longer-lived endogenous antimicrobial componentproduced during oxidative burst by neutrophils and monocytes.
Stability of NVC-422 in 10% synthetic tears containing lysozyme,IgG, albumin and other components was tested using HPLC withUV detection. Bacterial time kill kinetics was studied in 5mM acetate saline, pH 4 and in the presence of synthetic tears.Antiviral activity was determined by incubating viruses withserial dilutions of test compounds in 5 mM acetate saline, pH4 followed by cell infection. The titer of virus was calculatedbased on the number of wells that show cytopathic effect (CPE).The TCID50 was determined from the dilution where 50% of thecultures were positive for CPE.
NVC-422 showed stability in 10% synthetic tears (97.5% remaining)after a 2 hr incubation. 0.3% NVC-422 in acetate saline pH 4in the presence of synthetic tears was bacteriocidal againstGram-positive and Gram-negative bacteria (Table1). Antiviralactivity of NVC-422 was retained in 10% synthetic tears. 0.1%NVC-422 reduced all viral titers to the limit of detection (Table2). See tables below.
NVC-422 retains its chemical stability, virucidal and bactericidalactivity in presence of synthetic tears.
This PDF is available to Subscribers Only