April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Antiviral Effect of N-Chlorotaurine Against Adenovirus in vitro in Serotypes Inducing Keratoconjunctivitis
Author Affiliations & Notes
  • J. Y. Huang
    Ophthalmology, Fukuoka Univ School of Medicine, Fukuoka, Japan
  • E. Uchio
    Ophthalmology, Fukuoka Univ School of Medicine, Fukuoka, Japan
  • H. Inoue
    Ophthalmology, Fukuoka Univ School of Medicine, Fukuoka, Japan
  • K. Kadonosono
    Ophthalmology, Yokohama City University Medical Center, Yokohama, Japan
  • H. Ishiko
    Central Laboratories, Mitsubishi Chemical Medience, Inc., Tokyo, Japan
  • Footnotes
    Commercial Relationships  J.Y. Huang, None; E. Uchio, None; H. Inoue, None; K. Kadonosono, None; H. Ishiko, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 1994. doi:
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      J. Y. Huang, E. Uchio, H. Inoue, K. Kadonosono, H. Ishiko; Antiviral Effect of N-Chlorotaurine Against Adenovirus in vitro in Serotypes Inducing Keratoconjunctivitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1994.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : N-chlorotaurine (Cl-HN-CH2-CH2-SO3H, NCT) is the N-chloro derivative of the amino acid taurine, which is an oxidant produced by human granulocytes and monocytes during inflammatory reactions. We evaluated the anti-adenoviral effect of NCT by direct quantitative polymerase chain reaction (PCR) method in vitro in this study.

Methods: : After calculating CC50 (50% cytotoxic concentration) of NCT against A549 cells by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, adenovirus (AdV) serotypes 3, 4, 8, 19 and 37 were cultured with NCT for seven days, and the extracted adenoviral DNA was quantitatively measured by real-time PCR.

Results: : NCT showed an inhibitory effect against adenoviral proliferation in all serotypes in a dose-dependent manner. Among the serotypes, NCT was particularly effective against AdV type 3 and serotypes of species D. The selective index of NCT for each AdV serotype ranged between 11.5 and 60.2.

Conclusions: : This study showed that NCT has an antiviral effect against most serotypes of human AdV.

Keywords: adenovirus • conjunctivitis • antiviral drugs 
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