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J. Y. Huang, E. Uchio, H. Inoue, K. Kadonosono, H. Ishiko; Antiviral Effect of N-Chlorotaurine Against Adenovirus in vitro in Serotypes Inducing Keratoconjunctivitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):1994.
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N-chlorotaurine (Cl-HN-CH2-CH2-SO3H, NCT) is the N-chloro derivative of the amino acid taurine, which is an oxidant produced by human granulocytes and monocytes during inflammatory reactions. We evaluated the anti-adenoviral effect of NCT by direct quantitative polymerase chain reaction (PCR) method in vitro in this study.
After calculating CC50 (50% cytotoxic concentration) of NCT against A549 cells by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, adenovirus (AdV) serotypes 3, 4, 8, 19 and 37 were cultured with NCT for seven days, and the extracted adenoviral DNA was quantitatively measured by real-time PCR.
NCT showed an inhibitory effect against adenoviral proliferation in all serotypes in a dose-dependent manner. Among the serotypes, NCT was particularly effective against AdV type 3 and serotypes of species D. The selective index of NCT for each AdV serotype ranged between 11.5 and 60.2.
This study showed that NCT has an antiviral effect against most serotypes of human AdV.
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