Purchase this article with an account.
C. B. Toris, S. Fan, L. S. Stapp, T. L. Rudebush, G. Prasanna, S. Anderson, A. Krauss; Comparison of the Effects of an EP2, EP4 and EP2/4 Agonist on Aqueous Humor Dynamics in Monkeys. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2007.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The purpose of this study was to determine the effects of an EP2, EP4 and a mixed EP2/4 agonist on intraocular pressure (IOP) and aqueous humor dynamics in monkeys.
Three masked, crossover experiments were performed in 12 to 14 female cynomolgus monkeys with unilateral laser-induced glaucoma. Seated intraocular pressures were measured in conscious animals by pneumatonometry at 9AM on baseline day (IOP1). Eyes were dosed once-daily for three days with agonists of EP2 (0.01%), EP4 (0.00025%) or EP2-and-4 (0.0005%) receptors, or vehicle. Measurements on day 4 of dosing were made in ketamine sedated animals. These included IOPs by pneumatonometry at 9AM (IOP2) and noon (IOP3), aqueous flow (Fa, µl/min) by fluorophotometry, outflow facility (µl/min/mmHg) by fluorophotometry (Cfl) and by 2-minute tonography (Cton), and uveoscleral outflow (Fufl or Futon, µl/min) by mathematical calculation.
Results are summarized in Table. Values are Means±SD. Drug treatments and corresponding vehicle treatments were compared by two-tailed paired t-tests (p).
The EP2, EP4 and mixed EP2/4 agonists in this study reduced IOP by increasing uveoscleral outflow. The two determinations of uveoscleral outflow (Fufl and Futon) showed increases of at least 1.2-fold with drug treatment compared to vehicle treatment, however these findings were not always statistically significant. Unlike another study of a different EP4 agonist, outflow facility was unchanged by the EP4 agonist in the current study.
This PDF is available to Subscribers Only